ArticleAnalytical chemistry2013
Single cell antimicrobial susceptibility testing by confined microchannels and electrokinetic loading.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 109 citations in OpenAlex.
- Artificial Intelligence-Empowered Single-Cell Phenotyping for Rapid, Automated Pathogen Diagnostics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Microfluidic point-of-care testing for antimicrobial resistance: advances, strategies, and One Health perspectives.Science in One Health · 2026Review
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- Precision single cell analysis to characterize host dependent antimicrobial response heterogeneity in physiological medium.Lab on a chip · 2025Article
- Microfluidic technologies for advanced antimicrobial susceptibility testing.Biomicrofluidics · 2024Review
- Growth Independent Morphometric Machine Learning Workflow for Single-Cell Antimicrobial Susceptibility Testing ofFrontiers in imaging (Lausanne, Switzerland) · 2024Article
- Accurate Prediction of Antimicrobial Susceptibility for Point-of-Care Testing of Urine in Less than 90 Minutes via iPRISM Cassettes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Single-cell pathogen diagnostics for combating antibiotic resistance.Nature reviews. Methods primers · 2023Article
- Organ Chips and Visualization of Biological Systems.Advances in experimental medicine and biology · 2023Article
- Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium.Journal of visualized experiments : JoVE · 2022Article
- Microfluidic Systems for Antimicrobial Susceptibility Testing.Advances in biochemical engineering/biotechnology · 2022Article
- A Rapid Single-Cell Antimicrobial Susceptibility Testing Workflow for Bloodstream Infections.Biosensors · 2021Article
- A 3D-printed magnetic digital microfluidic diagnostic platform for rapid colorimetric sensing of carbapenemase-producingMicrosystems & nanoengineering · 2021Article
- Direct Antimicrobial Susceptibility Testing on Clinical Urine Samples by Optical Tracking of Single Cell Division Events.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Rapid Determination of Antimicrobial Susceptibility by Stimulated Raman Scattering Imaging of DAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Current state of the art in rapid diagnostics for antimicrobial resistance.Lab on a chip · 2020Review
- Capillary-Driven Flow Microfluidics Combined with Smartphone Detection: An Emerging Tool for Point-of-Care Diagnostics.Diagnostics (Basel, Switzerland) · 2020Review
- Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level.Chemical science · 2020Review
- Article
- 3D microfluidic gradient generator for combination antimicrobial susceptibility testing.Microsystems & nanoengineering · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Multidrug-resistant pathogens are an emerging global health problem. In addition to the need of developing new antibiotics in the pipeline, the ability to rapidly determine the antibiotic resistance profiles of bacteria represents one of the most crucial steps toward the management of infectious diseases and the prevention of multidrug-resistant pathogens. Here, we report a single cell antimicrobial susceptibility testing (AST) approach for rapid determination of the antibiotic resistance of bacterial pathogens. By confining individual bacteria in gas permeable microchannels with dimensions comparable to a single bacterium, the antibiotic resistance of the bacteria can be monitored in real-time at the single cell level. To facilitate the dynamic loading of the bacteria into the confined microchannels for observation, AC electrokinetics is demonstrated for capturing bacteria to defined locations in high-conductivity AST buffer. The electrokinetic technique achieves a loading efficiency of about 75% with a negligible effect on the bacterial growth rate. To optimize the protocol for single cell AST, the bacterial growth rate of individual bacteria under different antibiotic conditions has been determined systematically. The applicability of single cell AST is demonstrated by the rapid determination of the antimicrobial resistant profiles of uropathogenic clinical isolates in Mueller-Hinton media and in urine. The antibiotic resistance profiles of bacteria can be determined in less than 1 h compared to days in standard culture-based AST techniques.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.