ArticleACS nano2013
Sampling a biomarker of the human immunodeficiency virus across a synthetic nanopore.
Article in ACS nano, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Analytical Assessment of Hyaluronan and Its Heavy Chain Modification with Solid-State Nanopore Sensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2026Review
- Single-Molecule Protein Profiling Using Nanopores and Dimeric Aptamer-Modified DNA Carriers.Angewandte Chemie (International ed. in English) · 2025Article
- Nanopore-Based Neurotransmitter Detection: Advances, Challenges, and Future Perspectives.ACS nano · 2025Review
- Impact of nanotechnology on conventional and artificial intelligence-based biosensing strategies for the detection of viruses.Discover nano · 2023Review
- Real-time label-free detection of dynamic aptamer-small molecule interactions using a nanopore nucleic acid conformational sensor.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Nanopore Single-molecule Analysis of Biomarkers: Providing Possible Clues to Disease Diagnosis.Trends in analytical chemistry : TRAC · 2023Article
- Aptamers in Virology-A Consolidated Review of the Most Recent Advancements in Diagnosis and Therapy.Pharmaceutics · 2021Review
- Direct Detection of Conserved Viral Sequences and Other Nucleic Acid Motifs with Solid-State Nanopores.ACS nano · 2021Article
- Sensing with Nanopores and Aptamers: A Way Forward.Sensors (Basel, Switzerland) · 2020Review
- Application of Aptamers in Virus Detection and Antiviral Therapy.Frontiers in microbiology · 2019Review
- Single-molecule protein sensing in a nanopore: a tutorial.Chemical Society reviews · 2018Review
- Stochastic sensing of Angiotensin II with lysenin channels.Scientific reports · 2017Article
- The Transmembrane Domain of a Bicomponent ABC Transporter Exhibits Channel-Forming Activity.ACS chemical biology · 2016Article
- Fingerprinting of Peptides with a Large Channel of Bacteriophage Phi29 DNA Packaging Motor.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Yeast V-ATPase Proteolipid Ring Acts as a Large-conductance Transmembrane Protein Pore.Scientific reports · 2016Article
- Studies of RNA Sequence and Structure Using Nanopores.Progress in molecular biology and translational science · 2016Review
- Global redesign of a native β-barrel scaffold.Biochimica et biophysica acta · 2016Article
- Aptasensors for viral diagnostics.Trends in analytical chemistry : TRAC · 2015Review
- Selectively Sized Graphene-Based Nanopores for in Situ Single Molecule Sensing.ACS applied materials & interfaces · 2015Article
- Direct Sensing and Discrimination among Ubiquitin and Ubiquitin Chains Using Solid-State Nanopores.Biophysical journal · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
One primary goal in nanobiotechnology is designing new methodologies for molecular biomedical diagnosis at stages much earlier than currently possible and without use of expensive reagents and sophisticated equipment. In this work, we show the proof of principle for single-molecule detection of the nucleocapsid protein 7 (NCp7), a protein biomarker of the HIV-1 virus, using synthetic nanopores and the resistive-pulse technique. The biosensing mechanism relied upon specific interactions between NCp7 and aptamers of stem-loop 3 (SL3) in the packaging domain of the retroviral RNA genome. One critical step of this study was the choice of the optimal size of the nanopores for accurate, label-free determinations of the dissociation constant of the NCp7 protein-SL3 RNA aptamer complex. Therefore, we systematically investigated the NCp7 protein-SL3 RNA aptamer complex employing two categories of nanopores in a silicon nitride membrane: (i) small, whose internal diameter was smaller than 6 nm, and (ii) large, whose internal diameter was in the range of 7 to 15 nm. Here, we demonstrate that only the use of nanopores with an internal diameter that is smaller than or comparable with the largest cross-sectional size of the NCp7-SL3 aptamer complex enables accurate measurement of the dissociation constant between the two interacting partners. Notably, this determination can be accomplished without the need for prior nanopore functionalization. Moreover, using small solid-state nanopores, we demonstrate the ability to detect drug candidates that inhibit the binding interactions between NCp7 and SL3 RNA by using a test case of N-ethylmaleimide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.