Evidence map›Paper›PMID 23444842›Full record

ArticleBMC biochemistry2013

Expression, purification and biochemical characterization of Schizosaccharomyces pombe Mcm4, 6 and 7.

Meng Xu, Y Paul Chang, Xiaojiang S Chen

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Meng XuGraduate Program in Genetics, Molecular and Cell Biology, University of Southern California, Los Angeles, CA 90089, USA.
Y Paul Chang
Xiaojiang S Chen
University of Southern California · USUSC Norris Comprehensive Cancer Center · US

Funding

SV40 T Antigen Structure and Helicase MechanismsR01AI055926 · NIAID · UNIVERSITY OF SOUTHERN CALIFORNIA · PI CHEN, XIAOJIANG S · 2004 to 2013
$4.0M
Structural Studies of MCM ComplexR01GM080338 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI CHEN, XIAOJIANG S · 2009 to 2012
$1.4M
NIAID NIH HHS R01 AI055926NIGMS NIH HHS GM080338NIGMS NIH HHS R01 GM080338
6 · The paper itself

Abstract

backgroundThe hetero-hexamer of the eukaryotic minichromosome maintenance (MCM) proteins plays an essential role in replication of genomic DNA. The ring-shaped Mcm2-7 hexamers comprising one of each subunit show helicase activity in vitro, and form double-hexamers on DNA. The Mcm4/6/7 also forms a hexameric complex with helicase activity in vitro.

resultsWe used an Escherichiai coli expression system to express various domains of Schizosaccharomyces pombe Mcm4, 6 and 7 in order to characterize their domain structure, oligomeric states, and possible inter-/intra-subunit interactions. We also successfully employed a co-expression system to express Mcm4/6/7 at the same time in Escherichiai coli, and have purified functional Mcm4/6/7 complex in a hexameric state in high yield and purity, providing a means for generating large quantity of proteins for future structural and biochemical studies.

conclusionsBased on our results and those of others, models were proposed for the subunit arrangement and architecture of both the Mcm4/6/7 hexamer and the Mcm2-7 double-hexamer.

Indexed as

Gene Expression Regulation, FungalSchizosaccharomyces pombe ProteinsCell Cycle ProteinsDNA-Binding ProteinsEscherichia coliMinichromosome Maintenance Complex Component 4Minichromosome Maintenance Complex Component 6Minichromosome Maintenance Complex Component 7Protein BindingProtein MultimerizationProtein SubunitsRecombinant ProteinsSchizosaccharomycesCell Cycle ProteinsDNA-Binding Proteinsmcm4 protein, S pombemcm6 protein, S pombeMcm7 protein, S pombeMinichromosome Maintenance Complex Component 4Minichromosome Maintenance Complex Component 6Minichromosome Maintenance Complex Component 7Protein SubunitsRecombinant ProteinsSchizosaccharomyces pombe Proteins

Identifiers

PMID23444842
PMCPMC3605359
OpenAlexW2167347879

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.