ArticleCroatian medical journal2013
Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents.
Article in Croatian medical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 22 citations in OpenAlex.
- High-resolution hybrid micro-CT imaging pipeline for mouse brain region segmentation and volumetric morphometry.PloS one · 2024Article
- High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models.Scientific reports · 2022Article
- Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system.Scientific reports · 2021Article
- Micro-computed tomography: a new diagnostic tool in postmortem assessment of brain anatomy in small fetuses.Neuroradiology · 2019Article
- IRE1α inhibition decreased TXNIP/NLRP3 inflammasome activation through miR-17-5p after neonatal hypoxic-ischemic brain injury in rats.Journal of neuroinflammation · 2018Article
- Improvement of spatial learning and memory, cortical gyrification patterns and brain oxidative stress markers in diabetic rats treated withJournal of traditional and complementary medicine · 2018Article
- Micro-computed tomography in murine models of cerebral cavernous malformations as a paradigm for brain disease.Journal of neuroscience methods · 2016Article
- Potential and limitations of X-Ray micro-computed tomography in arthropod neuroanatomy: a methodological and comparative survey.The Journal of comparative neurology · 2015Article
- Delayed remote ischemic postconditioning improves long term sensory motor deficits in a neonatal hypoxic ischemic rat model.PloS one · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
aimTo explore the possibility of brain imaging by microcomputed tomography (microCT) using x-ray contrasting methods to visualize mouse brain ischemic lesions after middle cerebral artery occlusion (MCAO).
methodsIsolated brains were immersed in ionic or nonionic radio contrast agent (RCA) for 5 days and subsequently scanned using microCT scanner. To verify whether ex-vivo microCT brain images can be used to characterize ischemic lesions, they were compared to Nissl stained serial histological sections of the same brains. To verify if brains immersed in RCA may be used afterwards for other methods, subsequent immunofluorescent labeling with anti-NeuN was performed.
resultsNonionic RCA showed better gray to white matter contrast in the brain, and therefore was selected for further studies. MicroCT measurement of ischemic lesion size and cerebral edema significantly correlated with the values determined by Nissl staining (ischemic lesion size: P=0.0005; cerebral edema: P=0.0002). Brain immersion in nonionic RCA did not affect subsequent immunofluorescent analysis and NeuN immunoreactivity.
conclusionMicroCT method was proven to be suitable for delineation of the ischemic lesion from the non-infarcted tissue, and quantification of lesion volume and cerebral edema.
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