ArticleNucleic acids research2013
The MASTER (methylation-assisted tailorable ends rational) ligation method for seamless DNA assembly.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- UniClo technology exploits methylation for universal scarless DNA assembly.Biology methods & protocols · 2026Review
- CRISPR/Cas system-guided plasmid mutagenesis without sequence restriction.Fundamental research · 2025Article
- UniClo: scarless hierarchical DNA assembly without sequence constraint.Nucleic acids research · 2025Article
- Recent advances in the direct cloning of large natural product biosynthetic gene clusters.Engineering microbiology · 2023Review
- PlasmidMaker is a versatile, automated, and high throughput end-to-end platform for plasmid construction.Nature communications · 2022Article
- Recent Advances in Strategies for the Cloning of Natural Product Biosynthetic Gene Clusters.Frontiers in bioengineering and biotechnology · 2021Review
- Article
- Recent advances in natural products exploitation inEngineering in life sciences · 2019Review
- A seamless and iterative DNA assembly method named PS-Brick and its assisted metabolic engineering for threonine and 1-propanol production.Biotechnology for biofuels · 2019Article
- Recent Advances in Synthetic Biology Approaches to Optimize Production of Bioactive Natural Products in Actinobacteria.Frontiers in microbiology · 2019Review
- Polymorphism studies on microRNA targetome of thalassemia.Bioinformation · 2018Article
- The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro.Nucleic acids research · 2017Article
- Scarless assembly of unphosphorylated DNA fragments with a simplified DATEL method.Bioengineered · 2017Article
- BioBrick-based 'Quick Gene Assembly'Synthetic biology (Oxford, England) · 2017Article
- Next generation gene synthesis: From microarrays to genomes.Engineering in life sciences · 2017Review
- Engineering microbial hosts for production of bacterial natural products.Natural product reports · 2016Review
- High-throughput recombinant protein expression in Escherichia coli: current status and future perspectives.Open biology · 2016Review
- Synthetic biology to access and expand nature's chemical diversity.Nature reviews. Microbiology · 2016Review
- New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters.Natural product reports · 2016Review
- Bricks and blueprints: methods and standards for DNA assembly.Nature reviews. Molecular cell biology · 2015Review
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4 authors.
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Abstract
Techniques for assembly of designed DNA sequences are important for synthetic biology. So far, a few methods have been developed towards high-throughput seamless DNA assembly in vitro, including both the homologous sequences-based system and the type IIS-mediated system. Here, we describe a novel method designated 'MASTER Ligation', by which multiple DNA sequences can be seamlessly assembled through a simple and sequence-independent hierarchical procedure. The key restriction endonuclease used, MspJI, shares both type IIM and type IIS properties; thus, it only recognizes the methylation-specific 4-bp sites, (m)CNNR (R = A or G), and cuts DNA outside of the recognition sequences. This method was tested via successful assembly of either multiple polymerase chain reaction amplicons or restriction fragments of the actinorhodin biosynthetic cluster of Streptomyces coelicolor (∼29 kb), which was further heterologously expressed in a fast-growing and moderately thermophilic strain, Streptomyces sp. 4F.
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