ArticleThe Journal of biological chemistry2013
Nicotinamide inhibits the lysosomal cathepsin b-like protease and kills African trypanosomes.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Niacinamide modulation by hemocyanin shapes hemolymph microbial communities in Penaeid shrimp.ISME communications · 2026Article
- Nicotinamide mitigates visceral leishmaniasis by regulating inflammatory response and enhancing lipid metabolism.Parasites & vectors · 2024Article
- Targeting Cysteine Proteases and their Inhibitors to Combat Trypanosomiasis.Current medicinal chemistry · 2024Review
- The dawn of repurposing vitamins as potential novel antimicrobial agents: A call for global emergency response amidst AMR crisis.Health science reports · 2023Article
- Succinylation modification provides new insights for the treatment of immunocompromised individuals with drug-resistantFrontiers in immunology · 2023Article
- Curvicollide D Isolated from the FungusInternational journal of molecular sciences · 2022Article
- [Inhibitory Effects of Nicotinamide onSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2022Article
- Nicotinamide could reduce growth and cariogenic virulence ofJournal of oral microbiology · 2022Article
- Effect of Nicotinamide AgainstFrontiers in microbiology · 2019Article
- Inhibitors of Trypanosoma cruzi Sir2 related protein 1 as potential drugs against Chagas disease.PLoS neglected tropical diseases · 2018Article
- The superfamily keeps growing: Identification in trypanosomatids of RibJ, the first riboflavin transporter family in protists.PLoS neglected tropical diseases · 2017Article
- Specific Cell Targeting Therapy Bypasses Drug Resistance Mechanisms in African Trypanosomiasis.PLoS pathogens · 2015Article
- Amide-controlled, one-pot synthesis of tri-substituted purines generates structural diversity and analogues with trypanocidal activity.Scientific reports · 2015Article
- Tryps and trips: cell trafficking across the 100-year-old blood-brain barrier.Trends in neurosciences · 2014Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotinamide, a soluble compound of the vitamin B3 group, has antimicrobial activity against several microorganisms ranging from viruses to parasite protozoans. However, the mode of action of this antimicrobial activity is unknown. Here, we investigate the trypanocidal activity of nicotinamide on Trypanosoma brucei, the causative agent of African trypanosomiasis. Incubation of trypanosomes with nicotinamide causes deleterious defects in endocytic traffic, disruption of the lysosome, failure of cytokinesis, and, ultimately, cell death. At the same concentrations there was no effect on a cultured mammalian cell line. The effects on endocytosis and vesicle traffic were visible within 3 h and can be attributed to inhibition of lysosomal cathepsin b-like protease activity. The inhibitory effect of nicotinamide was confirmed by a direct activity assay of recombinant cathepsin b-like protein. Taken together, these data demonstrate that inhibition of the lysosomal protease cathepsin b-like blocks endocytosis, causing cell death. In addition, these results demonstrate for the first time the inhibitory effect of nicotinamide on a protease.
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