ArticleThe Biochemical journal1990
Partial purification and properties of an AMP-specific soluble 5'-nucleotidase from pigeon heart.
Article in The Biochemical journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 79 citations in OpenAlex.
- Efficient Gene Expression System in Medaka Embryos Enables Functional Characterization of nt5c1a Paralogs Involved in Inosine Monophosphate Metabolism.Genesis (New York, N.Y. : 2000) · 2026Article
- Metabolic Aspects of Adenosine Functions in the Brain.Frontiers in pharmacology · 2021Review
- The Inside Story of Adenosine.International journal of molecular sciences · 2018Review
- Evidence for control of adenosine metabolism in rat oxidative skeletal muscle by changes in pH.The Journal of physiology · 2000Article
- Structure-activity relationship of cytoplasmic 5'-nucleotidase substrate sites.The Biochemical journal · 1996Article
- Reconstitution of 5'-nucleotidase of bull seminal plasma in spin-labeled liposomes.The Journal of membrane biology · 1994Article
- Purification and some properties of an IMP-specific 5'-nucleotidase from yeast.The Biochemical journal · 1994Article
- Acidosis during ischemia promotes adenosine triphosphate resynthesis in postischemic rat heart. In vivo regulation of 5'-nucleotidase.The Journal of clinical investigation · 1994Article
- Effect of 5'-deoxy-5'-isobutylthioadenosine on formation and release of adenosine from neonatal and adult rat ventricular myocytes.The Biochemical journal · 1993Article
- Mechanisms of elevation of adenosine levels in anoxic hepatocytes.The Biochemical journal · 1993Article
- 5'-Nucleotidase: molecular structure and functional aspects.The Biochemical journal · 1992Review
- The influence of calcium antagonists on the adenine nucleotide metabolism in the guinea-pig working heart during ischaemia and reperfusion.Naunyn-Schmiedeberg's archives of pharmacology · 1991Article
- A soluble 5'-nucleotidase in rat kidney. Stimulation by decavanadate.The Biochemical journal · 1991Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A soluble 5'-nucleotidase was purified 200-fold from pigeon heart. The enzyme (1) had an apparent molecular mass close to 150 kDa, (2) had a neutral pH optimum and hydrolysed a wide range of nucleoside 5'-monophosphates with a 15-fold preference for AMP over IMP, (3) at near-physiological concentrations of AMP was activated by ADP but not by ATP, (4) was inhibited by high Mg2+ concentration and high ionic strength, (5) was weakly inhibited by p-nitrophenol phosphate and Pi, and (6) was non-competitively inhibited more potently by 5'-deoxy-5'-isobutylthioinosine than by 5'-deoxy-5'-isobutylthioadenosine, but not by [alpha,beta-methylene]ADP. The data show that the enzyme is distinct from the ecto-5'-nucleotidase and from the previously purified IMP-specific 5'-nucleotidase. They also predict that the enzyme is activated during ATP catabolism and hence will generate a more-than-linear increase in the adenosine-formation rate in response to an increase in cytosolic AMP concentration.
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