ArticleBiophysical journal2013
Intramolecular proton transfer in channelrhodopsins.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 69 citations in OpenAlex.
- Channel gating in bacteriorhodopsin-like channelrhodopsins with contrasting KBiophysical journal · 2026Article
- A red-light gate for a cation channel: The conducting state of channelrhodopsin-1 from Chlamydomonas augustae.Biophysical journal · 2026Article
- Multicolor photoreactions of the red light-activated channelrhodopsin Chrimson.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Sensing a rainbow of colors: algal photoreceptors.Frontiers in plant science · 2025Review
- Sodium-Selective Channelrhodopsins.Cells · 2024Review
- Optogenetics: Illuminating the Future of Hearing Restoration and Understanding Auditory Perception.Current gene therapy · 2024Review
- Sequential absorption of two photons creates a bistable form of RubyACR responsible for its strong desensitization.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Potassium-selective channelrhodopsins.Biophysics and physicobiology · 2023Article
- Time-resolved photoacoustics of channelrhodopsins: early energetics and light-driven volume changes.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- Reaction Dynamics in the Chrimson Channelrhodopsin: Observation of Product-State Evolution and Slow Diffusive Protein Motions.The journal of physical chemistry letters · 2023Article
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- Kalium channelrhodopsins are natural light-gated potassium channels that mediate optogenetic inhibition.Nature neuroscience · 2022Article
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- The effect on ion channel of different protonation states of E90 in channelrhodopsin-2: a molecular dynamics simulation.RSC advances · 2021Article
- RubyACRs, nonalgal anion channelrhodopsins with highly red-shifted absorption.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Conductance Mechanisms of Rapidly Desensitizing Cation Channelrhodopsins from Cryptophyte Algae.mBio · 2020Article
- Unifying photocycle model for light adaptation and temporal evolution of cation conductance in channelrhodopsin-2.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Mechanism by which water and protein electrostatic interactions control proton transfer at the active site of channelrhodopsin.PloS one · 2018Article
- Bacteriorhodopsin-like channelrhodopsins: Alternative mechanism for control of cation conductance.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
Channelrhodopsins serve as photoreceptors that control the motility behavior of green flagellate algae and act as light-gated ion channels when heterologously expressed in animal cells. Here, we report direct measurements of proton transfer from the retinylidene Schiff base in several channelrhodopsin variants expressed in HEK293 cells. A fast outward-directed current precedes the passive channel current that has the opposite direction at physiological holding potentials. This rapid charge movement occurs on the timescale of the M intermediate formation in microbial rhodopsins, including that for channelrhodopsin from Chlamydomonas augustae and its mutants, reported in this study. Mutant analysis showed that the glutamate residue corresponding to Asp(85) in bacteriorhodopsin acts as the primary acceptor of the Schiff-base proton in low-efficiency channelrhodopsins. Another photoactive-site residue corresponding to Asp(212) in bacteriorhodopsin serves as an alternative proton acceptor and plays a more important role in channel opening than the primary acceptor. In more efficient channelrhodopsins from Chlamydomonas reinhardtii, Mesostigma viride, and Platymonas (Tetraselmis) subcordiformis, the fast current was apparently absent. The inverse correlation of the outward proton transfer and channel activity is consistent with channel function evolving in channelrhodopsins at the expense of their capacity for active proton transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.