ArticleBiophysical journal2013
Two-photon excitation STED microscopy in two colors in acute brain slices.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
- Dendritic shaft constrictions shape synaptic integration in neurons.Science advances · 2026Article
- Wavelength-tunable depletion expands fluorophore compatibility in two-photon STED nanoscopy.Biomedical optics express · 2026Article
- Interferometric ultra-high resolution 3D imaging through brain sections.Nature communications · 2026Article
- Live STED imaging of functional neuroanatomy.Nature protocols · 2025Review
- Understanding the nervous system: lessons from Frontiers in Neurophotonics.Neurophotonics · 2024Review
- Pushing the Resolution Limit of Stimulated Emission Depletion Optical Nanoscopy.International journal of molecular sciences · 2023Review
- Imaging dendritic spines in the hippocampus of a living mouse by 3D-stimulated emission depletion microscopy.Neurophotonics · 2023Article
- Advanced imaging techniques for tracking drug dynamics at the subcellular level.Advanced drug delivery reviews · 2023Review
- Fluorescence radial fluctuation enables two-photon super-resolution microscopy.Frontiers in cellular neuroscience · 2023Article
- Article
- Improving two-photon excitation microscopy for sharper and faster biological imaging.Biophysics and physicobiology · 2023Article
- Super-Resolution Microscopy Opens New Doors to Life at the Nanoscale.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Review
- Review
- Commercially derived versatile optical architecture for two-photon STED, wavelength mixing and label-free microscopy.Biomedical optics express · 2022Article
- Super-resolution imaging to reveal the nanostructure of tripartite synapses.Neuronal signaling · 2021Review
- From whole organism to ultrastructure: progress in axonal imaging for decoding circuit development.Development (Cambridge, England) · 2021Review
- Aberration correction in stimulated emission depletion microscopy to increase imaging depth in living brain tissue.Neurophotonics · 2021Article
- Epigenetics and Communication Mechanisms in Microglia Activation with a View on Technological Approaches.Biomolecules · 2021Review
- Super-Resolution Microscopy: Shedding New Light onFrontiers in chemistry · 2021Review
- Axial resolution improvement of two-photon microscopy by multi-frame reconstruction and adaptive optics.Biomedical optics express · 2020Article
3 more citing papers are in PubMed but not listed here.
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many cellular structures and organelles are too small to be properly resolved by conventional light microscopy. This is particularly true for dendritic spines and glial processes, which are very small, dynamic, and embedded in dense tissue, making it difficult to image them under realistic experimental conditions. Two-photon microscopy is currently the method of choice for imaging in thick living tissue preparations, both in acute brain slices and in vivo. However, the spatial resolution of a two-photon microscope, which is limited to ~350 nm by the diffraction of light, is not sufficient for resolving many important details of neural morphology, such as the width of spine necks or thin glial processes. Recently developed superresolution approaches, such as stimulated emission depletion microscopy, have set new standards of optical resolution in imaging living tissue. However, the important goal of superresolution imaging with significant subdiffraction resolution has not yet been accomplished in acute brain slices. To overcome this limitation, we have developed a new microscope based on two-photon excitation and pulsed stimulated emission depletion microscopy, which provides unprecedented spatial resolution and excellent experimental access in acute brain slices using a long-working distance objective. The new microscope improves on the spatial resolution of a regular two-photon microscope by a factor of four to six, and it is compatible with time-lapse and simultaneous two-color superresolution imaging in living cells. We demonstrate the potential of this nanoscopy approach for brain slice physiology by imaging the morphology of dendritic spines and microglial cells well below the surface of acute brain slices.
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