ArticleBiophysical journal2013
Correlated spatio-temporal fluctuations in chromatin compaction states characterize stem cells.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 76 citations in OpenAlex.
- Differential Crosslinking and Contractile Motors Drive Nuclear Chromatin Compaction.bioRxiv : the preprint server for biology · 2025Article
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- Interplay of chromatin organization and mechanics of the cell nucleus.Biophysical journal · 2024Article
- Unsupervised representation learning of chromatin images identifies changes in cell state and tissue organization in DCIS.Nature communications · 2024Article
- Transient crosslinking controls the condensate formation pathway within chromatin networks.Physical review. E · 2024Article
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- Global genome decompaction leads to stochastic activation of gene expression as a first step toward fate commitment in human hematopoietic cells.PLoS biology · 2022Article
- Actomyosin contractility as a mechanical checkpoint for cell state transitions.Scientific reports · 2022Article
- Nucleus-cytoskeleton communication impacts on OCT4-chromatin interactions in embryonic stem cells.BMC biology · 2022Article
- Nuclear envelope mechanobiology: linking the nuclear structure and function.Nucleus (Austin, Tex.) · 2021Review
- Interphase Chromatin Undergoes a Local Sol-Gel Transition upon Cell Differentiation.Physical review letters · 2021Article
- The characteristics of nuclear membrane fluctuations in stem cells.Journal of the Royal Society, Interface · 2021Article
- The rich inner life of the cell nucleus: dynamic organization, active flows, and emergent rheology.Biophysical reviews · 2020Review
- KRAS-retroviral fusion transcripts and gene amplification in arsenic-transformed, human prostate CAsE-PE cancer cells.Toxicology and applied pharmacology · 2020Article
- Deformation Microscopy for Dynamic Intracellular and Intranuclear Mapping of Mechanics with High Spatiotemporal Resolution.Cell reports · 2019Article
- Nuclear morphometrics and chromatin condensation patterns as disease biomarkers using a mobile microscope.PloS one · 2019Article
- A microfluidic device for isolating intact chromosomes from single mammalian cells and probing their folding stability by controlling solution conditions.Scientific reports · 2018Article
- Emerin modulates spatial organization of chromosome territories in cells on softer matrices.Nucleic acids research · 2018Article
- Laterally confined growth of cells induces nuclear reprogramming in the absence of exogenous biochemical factors.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cells integrate signals from the microenvironment to generate lineage-specific gene expression programs upon differentiation. Undifferentiated cell nuclei are easily deformable, with an active transcriptome, whereas differentiated cells have stiffer nuclei and condensed chromatin. Chromatin organization in the stem cell state is known to be highly dynamic but quantitative characterizations of its plasticity are lacking. Using fluorescence imaging, we study the spatio-temporal dynamics of nuclear architecture and chromatin compaction in mouse embryonic stem (ES) cells and differentiated states. Individual ES cells exhibit a relatively narrow variation in chromatin compaction, whereas primary mouse embryonic fibroblasts (PMEF) show broad distributions. However, spatial correlations in chromatin compaction exhibit an emergent length scale in PMEFs, although they are unstructured and longer ranged in ES cells. We provide evidence for correlated fluctuations with large amplitude and long intrinsic timescales, including an oscillatory component, in both chromatin compaction and nuclear area in ES cells. Such fluctuations are largely frozen in PMEF. The role of actin and Lamin A/C in modulating these fluctuations is described. A simple theoretical formulation reproduces the observed dynamics. Our results suggest that, in addition to nuclear plasticity, correlated spatio-temporal structural fluctuations of chromatin in undifferentiated cells characterize the stem cell state.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.