ArticleBiophysical journal2013
Understanding the role of amphipathic helices in N-BAR domain driven membrane remodeling.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 86 citations in OpenAlex.
- The bacterial ESCRT-III PspA rods thin lipid tubules and increase membrane curvature through helix α0 interactions.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- GTP hydrolysis triggers membrane remodeling by AMPH-1.Science advances · 2025Article
- Atomistic Simulations and Analysis of Peripheral Membrane Proteins with Model Lipid Bilayers.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Statistical Mechanical Design Principles for Coarse-Grained Interactions across Different Conformational Free Energy Surfaces.The journal of physical chemistry letters · 2023Article
- Curvature dependence of BAR protein membrane association and dissociation kinetics.Scientific reports · 2022Article
- The endophilin curvature-sensitive motif requires electrostatic guidance to recycle synaptic vesicles in vivo.Developmental cell · 2022Article
- Article
- Coarse-Grained Force Fields from the Perspective of Statistical Mechanics: Better Understanding of the Origins of a MARTINI Hangover.Journal of chemical theory and computation · 2021Article
- Complimentary action of structured and unstructured domains of epsin supports clathrin-mediated endocytosis at high tension.Communications biology · 2020Article
- Mechanisms of negative membrane curvature sensing and generation by ESCRT III subunit Snf7.Protein science : a publication of the Protein Society · 2020Article
- Unusual Organization of I-BAR Proteins on Tubular and Vesicular Membranes.Biophysical journal · 2019Article
- ACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages.PLoS computational biology · 2019Article
- Curvature induction and membrane remodeling by FAM134B reticulon homology domain assist selective ER-phagy.Nature communications · 2019Article
- Multiconfigurational Coarse-Grained Molecular Dynamics.Journal of chemical theory and computation · 2019Article
- Characterization of Lipid-Protein Interactions and Lipid-Mediated Modulation of Membrane Protein Function through Molecular Simulation.Chemical reviews · 2019Review
- Directed Supramolecular Organization of N-BAR Proteins through Regulation of H0 Membrane Immersion Depth.Scientific reports · 2018Article
- Long-Range Organization of Membrane-Curving Proteins.ACS central science · 2017Article
- Salt Bridge Formation between the I-BAR Domain and Lipids Increases Lipid Density and Membrane Curvature.Scientific reports · 2017Article
- Article
- The N-Terminal Amphipathic Helix of Endophilin Does Not Contribute to Its Molecular Curvature Generation Capacity.Journal of the American Chemical Society · 2016Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Endophilin N-BAR (N-terminal helix and Bin/amphiphysin/Rvs) domain tubulates and vesiculates lipid membranes in vitro via its crescent-shaped dimer and four amphipathic helices that penetrate into membranes as wedges. Like F-BAR domains, endophilin N-BAR also forms a scaffold on membrane tubes. Unlike F-BARs, endophilin N-BARs have N-terminal H0 amphipathic helices that are proposed to interact with other N-BARs in oligomer lattices. Recent cryo-electron microscopy reconstructions shed light on the organization of the N-BAR lattice coats on a nanometer scale. However, because of the resolution of the reconstructions, the precise positioning of the amphipathic helices is still ambiguous. In this work, we applied a coarse-grained model to study various membrane remodeling scenarios induced by endophilin N-BARs. We found that H0 helices of N-BARs prefer to align in an antiparallel manner at two ends of the protein to form a stable lattice. The deletion of H0 helices causes disruption of the lattice. In addition, we analyzed the persistence lengths of the protein-coated tubes and found that the stiffness of endophilin N-BAR-coated tubules qualitatively agrees with previous experimental work studying N-BAR-coated tubules. Large-scale simulations on membrane liposomes revealed a systematic relation between H0 helix density and local membrane curvature fluctuations. The data also suggest that the H0 helix is required for BARs to form organized structures on the liposome, further illustrating its important function.
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