ArticleAnnals of clinical microbiology and antimicrobials2013
Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries.
Article in Annals of clinical microbiology and antimicrobials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers, 4 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
99 citing papers in PubMed, 4 syntheses or guidelines pooled it, 243 citations in OpenAlex.
- Antimicrobial effect of silver diamine fluoride (SDF) in arresting dentine caries of permanent teeth: A Systematic review.F1000Research · 2024Pooled it
- Pooled it
- Clinical efficacy and the antimicrobial potential of silver formulations in arresting dental caries: a systematic review.BMC oral health · 2020Pooled it
- Effect of Antimicrobial Interventions on the Oral Microbiota Associated with Early Childhood Caries.Pediatric dentistryPooled it
- Randomized Clinical Trial on Caries Prevention of Silver Diamine Fluoride.Journal of dental research · 2026Trial
- Caries Prevention Using Silver Diamine Fluoride: A 12-Month Clinical Trial.International dental journal · 2023Trial
- Management of initial carious lesions of hypomineralized molars (MIH) with silver diamine fluoride or silver-modified atraumatic restorative treatment (SMART): 1-year results of a prospective, randomized clinical trial.Clinical oral investigations · 2022Trial
- Three-Month Effect of Silver Diamine Fluoride (SDF) in Salivary Levels of Streptococcus Mutans in Children. An Exploratory Trial.Oral health & preventive dentistry · 2020Trial
- Topical silver diamine fluoride for dental caries arrest in preschool children: A randomized controlled trial and microbiological analysis of caries associated microbes and resistance gene expression.Journal of dentistry · 2018Trial
- Silver diamine fluoride and glass ionomer differentially remineralize early caries lesions, in situ.Clinical oral investigations · 2016Trial
- Knowledge and attitudes regarding silver diamine fluoride among Brazilian dentists: a cross-sectional study.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2026Article
- Article
- Efficacy of topical strategies to prevent the onset of root caries lesions: an in vitro study.Odontology · 2026Article
- Effect of ammonia-free silver fluoride application on the resin-dentin interface subjected to an in situ cariogenic challenge.Clinical oral investigations · 2026Article
- Assessment of tannic acid and glutathione in 38% silver diamine fluoride for dentine stain reduction with antibacterial efficacy: AnBioinformation · 2026Article
- Effect of nano silver fluoride pre-treatment on the microtensile bond strength of a universal adhesive to dentin: an in vitro study.Biomaterial investigations in dentistry · 2026Article
- The effect of using silver diamine fluoride at two different times on the microshear bond strength of adhesives to sound and caries-affected dentin.Dental research journal · 2026Article
- Antibacterial effects and remineralization potential of silver nanoparticles in a novel nanosilver fluoride formulation.BMC oral health · 2025Article
- In vitro evaluation of the effects of various remineralization agents on root caries.BMC oral health · 2025Article
- Comparative evaluation of 38% silver diamine fluoride on sealing ability of biodentine and MTA angelus as a perforation repair material: a confocal laser microscopic study.BMC oral health · 2025Article
39 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundsSilver diamine fluoride (SDF) has clinical success in arresting dentin caries, this study aimed to investigate its mechanism of action.
methodsUsing a computer-controlled artificial mouth, we studied the effect of 38% SDF on cariogenic biofilms and dentin carious lesions. We used five common cariogenic bacteria (Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lactobacillus rhamnosus and Actinomyces naeslundii) to form a cariogenic biofilm that generated carious lesions with a depth of approximately 70 um on human dentin blocks. We applied 38% SDF to the lesions in the test group and water to those in the control group. The blocks were incubated in the artificial mouth for 21 days before evaluation. Microbial kinetics, architecture, viability and distribution were evaluated every 7 days using colony forming unit (CFU), scanning electron microscopy and confocal laser scanning microscopy. The physical properties of the carious lesions were evaluated with microhardness testing, energy dispersive spectroscopy (EDS) and Fourier transform infra-red spectroscopy (FTIR).
resultsThe CFU results revealed fewer colony forming units in the test group compared with the control group (p < 0.01). Scanning electron microscopy and confocal microscopy showed less bacterial growth in the test group, and confluent cariogenic biofilm in the control group (p < 0.01). The microhardness and weight percentages of calcium and phosphorus in the test group from the outermost 50mum were higher than in the control group (p < 0.05). EDS showed that calcium and phosphous were higher in outer 50 mum in test groups than in the control FTIR revealed less exposed collagen I in the test lesions compared with the control group (p < 0.01).
conclusions38% SDF inhibits multi-species cariogenic biofilm formation on dentin carious lesions and reduces the demineralization process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.