ArticleBMC research notes2013
A fast and efficient method for preparation of high-quality RNA from fungal mycelia.
Article in BMC research notes, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 56 citations in OpenAlex.
- Total fungal RNA extraction from complex solid- state fermentation products.Applied microbiology and biotechnology · 2026Article
- Yeast-Fusarium interactions and mycotoxin modulation in the phyllosphere.The ISME journal · 2026Article
- Cinchona-based liquid formulation exhibits antifungal activity through Tryptophan starvation and disruption of mitochondrial respiration in Rhizoctonia Solani.Scientific reports · 2025Article
- Cuticular collagens mediate cross-kingdom predator-prey interactions between trapping fungi and nematodes.PLoS biology · 2025Article
- Transcriptomic Profiling of ThermotolerantJournal of fungi (Basel, Switzerland) · 2025Article
- Development of Biphasic Culture System for an Entomopathogenic FungusJournal of fungi (Basel, Switzerland) · 2025Article
- Comparative gene expression analysis ofPeerJ · 2025Article
- The nematode-trapping fungus Arthrobotrys oligospora detects prey pheromones via G protein-coupled receptors.Nature microbiology · 2024Article
- Validation of reference gene stability for normalization of RT-qPCR in Phytophthora capsici Leonian during its interaction with Piper nigrum L.Scientific reports · 2024Article
- Expanding the Toolbox for Genetic Manipulation inJournal of fungi (Basel, Switzerland) · 2024Article
- Dual-RNA-sequencing to elucidate the interactions between sorghum andFrontiers in fungal biology · 2024Article
- Transcriptomic analysis reveals pathogenicity mechanisms ofFrontiers in microbiology · 2024Article
- How Does the Sample Preparation ofCurrent issues in molecular biology · 2023Article
- Biochemical Evaluation ofMicroorganisms · 2022Article
- Characterizing the gene-environment interaction underlying natural morphological variation in Neurospora crassa conidiophores using high-throughput phenomics and transcriptomics.G3 (Bethesda, Md.) · 2022Article
- A modified culture medium and hyphae isolation method can increase quality of the RNA extracted from mycelia of a dimorphic fungal species.Current genetics · 2021Article
- Prey sensing and response in a nematode-trapping fungus is governed by the MAPK pheromone response pathway.Genetics · 2021Article
- In-Silico Characterization of Glycosyl Hydrolase Family 1 β-Glucosidase fromInternational journal of molecular sciences · 2020Article
- Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA.International journal of molecular sciences · 2020Review
- Determination of the effect of RBBR on laccase activity and gene expression level of fungi in lichen structure.3 Biotech · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFungal RNA samples are usually isolated from fungal mycelia grown in liquid culture, which relies on prolific growth of the fungus in liquid media. The fungal biomass is then collected by vacuum filtration, which can result in low recovery for samples with reduced biomass due to poor growth in liquid media.
findingsHere we report an alternative culturing method, based on growth on solid media which is independent of the ability of a fungus to grow in liquid culture. We show that growth on solid media overlayed with a nylon membrane is superior to other culturing methods, producing large amounts of biomass and allowing for easy harvesting of fungal mycelia. Furthermore, we show that mycelium harvested with this method yielded high-quality RNA, superior to RNA isolated from liquid grown mycelium. We also show that inclusion of a second chloroform extraction step in the procedure significantly increases RNA yield.
conclusionsThis method is particularly useful for fungal species that show poor or no growth in liquid media, but are easily cultured on solid media. Culturing can be performed on small petri dishes, which significantly reduces handling and therefore allowing growth and isolation of RNA from multiple strains in a high throughput manner. The obtained RNA samples are of high quality in sufficient quantities for several northern blot experiments or quantitative RT-PCR experiments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.