ArticleBMC microbiology2013
Detection limits of Legionella pneumophila in environmental samples after co-culture with Acanthamoeba polyphaga.
Article in BMC microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- A Biochemical Corrosion Monitoring Sensor with a Silver/Carbon Comb Structure for the Detection of LivingACS omega · 2023Article
- Detection and quantification of viable but non-culturableFrontiers in microbiology · 2023Article
- Use of a Novel DNA-Loaded Alginate-Calcium Carbonate Biopolymer Surrogate to Study the Engulfment of Legionella pneumophila by Acanthamoeba polyphaga in Water Systems.Microbiology spectrum · 2022Article
- Detection of Legionella species, the influence of precipitation on the amount of Legionella DNA, and bacterial microbiome in aerosols from outdoor sites near asphalt roads in Toyama Prefecture, Japan.BMC microbiology · 2021Article
- Detection ofMicroorganisms · 2018Article
- Adaptation of Amoeba Plate Test To Recover Legionella Strains from Clinical Samples.Journal of clinical microbiology · 2018Article
- Evaluation of Legionella Air Contamination in Healthcare Facilities by Different Sampling Methods: An Italian Multicenter Study.International journal of environmental research and public health · 2017Article
- Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review.Water research · 2017Review
- Legionella prevalence and risk of legionellosis in Japanese households.Epidemiology and infection · 2017Article
- Temperature-Dependent Growth Modeling of Environmental and Clinical Legionella pneumophila Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) Genotypes.Applied and environmental microbiology · 2017Article
- Molecular detection of Acanthamoeba spp., Naegleria fowleri and Vermamoeba (Hartmannella) vermiformis as vectors for Legionella spp. in untreated and solar pasteurized harvested rainwater.Parasites & vectors · 2016Article
- Optimized methods for Legionella pneumophila release from its Acanthamoeba hosts.BMC microbiology · 2016Article
- Influence of climate and geography on the occurrence of Legionella and amoebae in composting facilities.BMC research notes · 2014Article
- Presence of Legionella and free-living Amoebae in composts and bioaerosols from composting facilities.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe efficiency of recovery and the detection limit of Legionella after co-culture with Acanthamoeba polyphaga are not known and so far no investigations have been carried out to determine the efficiency of the recovery of Legionella spp. by co-culture and compare it with that of conventional culturing methods. This study aimed to assess the detection limits of co-culture compared to culture for Legionella pneumophila in compost and air samples. Compost and air samples were spiked with known concentrations of L. pneumophila. Direct culturing and co-culture with amoebae were used in parallel to isolate L. pneumophila and recovery standard curves for both methods were produced for each sample.
resultsThe co-culture proved to be more sensitive than the reference method, detecting 10²-10³ L. pneumophila cells in 1 g of spiked compost or 1 m³ of spiked air, as compared to 10⁵-10⁶ cells in 1 g of spiked compost and 1 m³ of spiked air.
conclusionsCo-culture with amoebae is a useful, sensitive and reliable technique to enrich L. pneumophila in environmental samples that contain only low amounts of bacterial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.