ArticleJournal of animal science and biotechnology2013
Deletions in the pyruvate pathway of Salmonella Typhimurium alter SPI1-mediated gene expression and infectivity.
Article in Journal of animal science and biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Immune protection and persistence in Pseudomonas aeruginosa infections by pyruvate cross-feeding.Nature communications · 2026Article
- Intracellular formate modulates a motility-invasion switch in Salmonella Typhimurium.PLoS pathogens · 2025Article
- Nitric Oxide Inhibition of Glycyl Radical Enzymes and Their Activases.Journal of the American Chemical Society · 2025Article
- Formate production is dispensable forInfection and immunity · 2023Article
- A novel, stain-free, natural auto-fluorescent signal, Sig M, identified from cytometric and transcriptomic analysis of infectivity ofFrontiers in cellular and infection microbiology · 2023Article
- The Biological Significance of Pyruvate Sensing and Uptake inMicroorganisms · 2022Article
- RNA atlas of human bacterial pathogens uncovers stress dynamics linked to infection.Nature communications · 2021Article
- Identification of common highly expressed genes of Salmonella Enteritidis by in silico prediction of gene expression and in vitro transcriptomic analysis.Poultry science · 2019Article
- The Transcription Factor ArcA ModulatesFrontiers in microbiology · 2019Article
- Methylthioadenosine Suppresses Salmonella Virulence.Infection and immunity · 2018Article
- Identification of new loci involved in the host susceptibility to Salmonella Typhimurium in collaborative cross mice.BMC genomics · 2018Article
- Article
- Exploiting a host-commensal interaction to promote intestinal barrier function and enteric pathogen tolerance.Science immunology · 2016Article
- The pyruvate-tricarboxylic acid cycle node: a focal point of virulence control in the enteric pathogen Yersinia pseudotuberculosis.The Journal of biological chemistry · 2014Article
- The metabolic enzyme AdhE controls the virulence of Escherichia coli O157:H7.Molecular microbiology · 2014Article
- Interrelationship between type three secretion system and metabolism in pathogenic bacteria.Frontiers in cellular and infection microbiology · 2014Review
- Nutritional and metabolic requirements for the infection of HeLa cells by Salmonella enterica serovar Typhimurium.PloS one · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSalmonella enterica serovar Typhimurium is a major foodborne pathogen worldwide. S. Typhimurium encodes type III secretion systems via Salmonella pathogenicity islands (SPI), producing the major effector proteins of virulence. Previously, we identified two genes of Salmonella pyruvate metabolism that were up-regulated during chicken cell infection: pyruvate formate lyase I (pflB) and bifunctional acetaldehyde-CoA/alcohol dehydrogenase (adhE). We were therefore interested in examining the role these genes may play in the transmission of Salmonella to humans.
methodsMutant strains of Salmonella with single gene deletions for pflB and adhE were created. Invasion and growth in human HCT-8 intestinal epithelial cells and THP-1 macrophages was examined. Quantitative PCR was performed on 19 SPI-1 genes.
resultsIn HCT-8 cells, both mutant strains had significantly higher intracellular counts than the wild-type from 4 to 48 h post-infection. Various SPI-1 genes in the mutants were up-regulated over the wild-type as early as 1 h and lasting until 24 h post-infection. In THP-1 cells, no significant difference in internal Salmonella counts was observed; however, SPI-1 genes were largely down-regulated in the mutants during the time-course of infection. We also found five SPI-1 genes - hilA, hilC hilD, sicP and rtsA - which were up-regulated in at least one of the mutant strains in log-phase broth cultures alone. We have therefore identified a set of SPI-1 virulence genes whose regulation is effected by the central metabolism of Salmonella.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.