ArticleThe journal of physical chemistry. B2013
Orthogonal spin labeling and Gd(III)-nitroxide distance measurements on bacteriophage T4-lysozyme.
Article in The journal of physical chemistry. B, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Characterization of flexible RNA binding by tandem RNA recognition motifs through integrative ensemble modelling.Nucleic acids research · 2026Article
- Exploring the dynamics and structure of PpiB in living Escherichia coli cells using electron paramagnetic resonance spectroscopy.Protein science : a publication of the Protein Society · 2024Article
- GdJournal of the American Chemical Society · 2023Article
- Increasing the Modulation Depth of GdThe journal of physical chemistry letters · 2022Article
- Site-selective generation of lanthanoid binding sites on proteins using 4-fluoro-2,6-dicyanopyridine.Magnetic resonance (Gottingen, Germany) · 2022Article
- In-Cell Characterization of the Stable Tyrosyl Radical in E. coli Ribonucleotide Reductase Using Advanced EPR Spectroscopy.Angewandte Chemie (International ed. in English) · 2021Article
- Substrate binding in the multidrug transporter MdfA in detergent solution and in lipid nanodiscs.Biophysical journal · 2021Article
- Isoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling through Cysteines and Alkyne-Bearing Noncanonical Amino Acids.Chembiochem : a European journal of chemical biology · 2020Article
- Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Strategies to identify and suppress crosstalk signals in double electron-electron resonance (DEER) experiments with gadoliniumMagnetic resonance (Gottingen, Germany) · 2020Article
- Article
- Sub-Micromolar Pulse Dipolar EPR Spectroscopy Reveals Increasing CuAngewandte Chemie (International ed. in English) · 2019Article
- Expanding the Genetic Code for Site-Directed Spin-Labeling.International journal of molecular sciences · 2019Review
- GdPhysical chemistry chemical physics : PCCP · 2017Article
- Reaching the quantum limit of sensitivity in electron spin resonance.Nature nanotechnology · 2016Article
- Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy.Journal of biomolecular NMR · 2015Article
- A chemically cross-linked knottin dimer binds integrins with picomolar affinity and inhibits tumor cell migration and proliferation.Journal of the American Chemical Society · 2015Article
- Zero field splitting fluctuations induced phase relaxation of Gd3+ in frozen solutions at cryogenic temperatures.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Article
- New developments in spin labels for pulsed dipolar EPR.Molecules (Basel, Switzerland) · 2014Review
- Copper-based pulsed dipolar ESR spectroscopy as a probe of protein conformation linked to disease states.Biophysical journal · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We present the first example of chemoselective site-specific spin labeling of a monomeric protein with two spectroscopically orthogonal spin labels: a gadolinium(III) chelate complex and a nitroxide radical. A detailed analysis of the performance of two commercially available Gd(III) ligands in the Gd(III)-nitroxide pulse double electron-electron resonance (DEER or PELDOR) experiment is reported. A modification of the flip angle of the pump pulse in the Gd(III)-nitroxide DEER experiment is proposed to optimize sensitivity.
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