ArticlePloS one2013
Magnetic separation and antibiotics selection enable enrichment of cells with ZFN/TALEN-induced mutations.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 60 citations in OpenAlex.
- Enrichment strategies to enhance genome editing.Journal of biomedical science · 2023Review
- Potentials, prospects and applications of genome editing technologies in livestock production.Saudi journal of biological sciences · 2022Review
- Rapid Assessment of CRISPR Transfection Efficiency and Enrichment of CRISPR Induced Mutations Using a Dual-Fluorescent Stable Reporter System.Frontiers in genome editing · 2022Article
- Magnetic-Activated Cell Sorting Using Coiled-Coil Peptides: An Alternative Strategy for Isolating Cells with High Efficiency and Specificity.ACS applied materials & interfaces · 2021Article
- Strategies for High-Efficiency Mutation Using the CRISPR/Cas System.Frontiers in cell and developmental biology · 2021Review
- HLA DR Genome Editing with TALENs in Human iPSCs Produced Immune-Tolerant Dendritic Cells.Stem cells international · 2021Article
- Hairless-knockout piglets generated using the clustered regularly interspaced short palindromic repeat/CRISPR-associated-9 exhibit abnormalities in the skin and thymus.Experimental animals · 2019Article
- Apancreatic pigs cloned using Pdx1-disrupted fibroblasts created via TALEN-mediated mutagenesis.Oncotarget · 2017Article
- An efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system.Cellular and molecular life sciences : CMLS · 2017Article
- Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering.eLife · 2017Article
- A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair.The Journal of biological chemistry · 2017Article
- Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis.Nature protocols · 2017Article
- Knockout of miR-221 and miR-222 reveals common and specific targets for paralogous miRNAs.RNA biology · 2017Article
- Enhancing Targeted Genomic DNA Editing in Chicken Cells Using the CRISPR/Cas9 System.PloS one · 2017Article
- Heroes of peer review: Hyongbum (Henry) Kim.Genome biology · 2016Article
- Generation of Human Embryonic Stem Cell Line Expressing zsGreen in Cholinergic Neurons Using CRISPR/Cas9 System.Neurochemical research · 2016Article
- Efficient Genome Editing in Chicken DF-1 Cells Using the CRISPR/Cas9 System.G3 (Bethesda, Md.) · 2016Article
- Re-evaluation of the roles of DROSHA, Export in 5, and DICER in microRNA biogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Recent developments and clinical studies utilizing engineered zinc finger nuclease technology.Cellular and molecular life sciences : CMLS · 2015Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to enrich cells with targeted mutations greatly facilitates the process of using engineered nucleases, including zinc-finger nucleases and transcription activator-like effector nucleases, to construct such cells. We previously used surrogate reporters to enrich cells containing nuclease-induced mutations via flow cytometry. This method is, however, limited by the availability of flow cytometers. Furthermore, sorted cells occasionally fail to form colonies after exposure to a strong laser and hydrostatic pressure. Here we describe two different types of novel reporters that enable mutant cell enrichment without the use of flow cytometers. We designed reporters that express H-2K(k), a surface antigen, and the hygromycin resistance protein (Hygro(R)), respectively, when insertions or deletions are generated at the target sequences by the activity of engineered nucleases. After cotransfection of these reporters and the engineered nuclease-encoding plasmids, H-2K(k)- and Hygro(R)-expressing cells were isolated using magnetic separation and hygromycin treatment, respectively. We found that mutant cells were drastically enriched in the isolated cells, suggesting that these two reporters enable efficient enrichment of mutants. We propose that these two reporters will greatly facilitate the use of engineered nucleases in a wider range of biomedical research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.