ArticlePloS one2013
Study on invadopodia formation for lung carcinoma invasion with a microfluidic 3D culture device.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 64 citations in OpenAlex.
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- Cancer cell dynamics on silica fibers.Scientific reports · 2025Article
- Happy feet: the key roles of podosomes and invadopodia in trophoblast invasion at the maternal-fetal interface.Frontiers in endocrinology · 2025Review
- Unveiling epithelial plasticity regulation in lung cancer: Exploring the cross-talk among Tks4 scaffold protein partners.Molecular biology of the cell · 2024Article
- Advances in Tumor Organoids for the Evaluation of Drugs: A Bibliographic Review.Pharmaceutics · 2022Review
- The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death.Pharmaceutics · 2022Article
- Visualizing cancer extravasation: from mechanistic studies to drug development.Cancer metastasis reviews · 2021Review
- Advancements in 3D Cell Culture Systems for Personalizing Anti-Cancer Therapies.Frontiers in oncology · 2021Review
- Microfluidic chips: recent advances, critical strategies in design, applications and future perspectives.Microfluidics and nanofluidics · 2021Review
- Cell Culture BasedFrontiers in bioengineering and biotechnology · 2020Review
- The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors.Materials (Basel, Switzerland) · 2019Review
- Microfluidics for studying metastatic patterns of lung cancer.Journal of nanobiotechnology · 2019Review
- Biofabrication strategies for creating microvascular complexity.Biofabrication · 2019Review
- Modelling cancer in microfluidic human organs-on-chips.Nature reviews. Cancer · 2019Review
- ERK phosphorylation functions in invadopodia formation in tongue cancer cells in a novel silicate fibre-based 3D cell culture system.International journal of oral science · 2018Article
- Oral inflammation promotes oral squamous cell carcinoma invasion.Oncotarget · 2018Article
- Inhibition of the Hedgehog Signaling Pathway Depresses the Cigarette Smoke-Induced Malignant Transformation of 16HBE Cells on a Microfluidic Chip.Chinese medical journal · 2018Article
- Microfluidics in Malignant Glioma Research and Precision Medicine.Advanced biosystems · 2018Article
- The Molecular Targets and Anti-Invasive Effects of 2,6-bis-(4-hydroxyl-3methoxybenzylidine) cyclohexanone or BHMC in MDA-MB-231 Human Breast Cancer Cells.Molecules (Basel, Switzerland) · 2018Article
- Epithelial-mesenchymal crosstalk influences cellular behavior in a 3D alveolus-fibroblast model system.Biomaterials · 2018Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Invadopodia or invasive feet, which are actin-rich membrane protrusions with matrix degradation activity formed by invasive cancer cells, are a key determinant in the malignant invasive progression of tumors and represent an important target for cancer therapies. In this work, we presented a microfluidic 3D culture device with continuous supplement of fresh media via a syringe pump. The device mimicked tumor microenvironment in vivo and could be used to assay invadopodia formation and to study the mechanism of human lung cancer invasion. With this device, we investigated the effects of epidermal growth factor (EGF) and matrix metalloproteinase (MMP) inhibitor, GM6001 on invadopodia formation by human non-small cell lung cancer cell line A549 in 3D matrix model. This device was composed of three units that were capable of achieving the assays on one control group and two experimental groups' cells, which were simultaneously pretreated with EGF or GM6001 in parallel. Immunofluorescence analysis of invadopodia formation and extracellular matrix degradation was conducted using confocal imaging system. We observed that EGF promoted invadopodia formation by A549 cells in 3D matrix and that GM6001 inhibited the process. These results demonstrated that epidermal growth factor receptor (EGFR) signaling played a significant role in invadopodia formation and related ECM degradation activity. Meanwhile, it was suggested that MMP inhibitor (GM6001) might be a powerful therapeutic agent targeting invadopodia formation in tumor invasion. This work clearly demonstrated that the microfluidic-based 3D culture device provided an applicable platform for elucidating the mechanism of cancer invasion and could be used in testing other anti-invasion agents.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.