ArticlePloS one2013
Operating Cooperatively (OC) sensor for highly specific recognition of nucleic acids.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Toward a Rational Approach to Design Split G-Quadruplex Probes.ACS chemical biology · 2019Article
- Divide and Control: Comparison of Split and Switch Hybridization Sensors.ChemistrySelect · 2017Article
- Thermodynamic framework to assess low abundance DNA mutation detection by hybridization.PloS one · 2017Article
- Nonequilibrium Hybridization Enables Discrimination of a Point Mutation within 5-40 °C.Journal of the American Chemical Society · 2016Article
- Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA.Scientific reports · 2015Article
- A differential fluorescent receptor for nucleic acid analysis.Chembiochem : a European journal of chemical biology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Molecular Beacon (MB) probes have been extensively used for nucleic acid analysis because of their ability to produce fluorescent signal in solution instantly after hybridization. The indirect binding of MB probe to a target analyte offers several advantages, including: improved genotyping accuracy and the possibility to analyse folded nucleic acids. Here we report on a new design for MB-based sensor, called 'Operating Cooperatively' (OC), which takes advantage of indirect binding of MB probe to a target analyte. The sensor consists of two unmodified DNA strands, which hybridize to a universal MB probe and a nucleic acid analyte to form a fluorescent complex. OC sensors were designed to analyze two human SNPs and E. coli 16S rRNA. High specificity of the approach was demonstrated by the detection of true analyte in over 100 times excess amount of single base substituted analytes. Taking into account the flexibility in the design and the simplicity in optimization, we conclude that OC sensors may become versatile and efficient tools for instant DNA and RNA analysis in homogeneous solution.
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Registered trials
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