ArticleMolecular vision2013
Stability of small non-coding RNA reference gene expression in the rat retina during exposure to cyclic hyperoxia.
Article in Molecular vision, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
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- Retinal biomarkers and pharmacological targets for Hermansky-Pudlak syndrome 7.Scientific reports · 2020Article
- Oxygen-induced circRNA profiles and coregulatory networks in a retinopathy of prematurity mouse model.Experimental and therapeutic medicine · 2019Article
- Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins.Scientific reports · 2018Article
- Evaluation of the plasma microRNA levels in stage 3 premature retinopathy with plus disease: preliminary study.Eye (London, England) · 2018Article
- Estimation of the human postmortem interval using an established rat mathematical model and multi-RNA markers.Forensic science, medicine, and pathology · 2017Article
- Retinal and Circulating miRNAs in Age-Related Macular Degeneration: AnFrontiers in pharmacology · 2017Article
- Biocompatibility of fluids for multiphase drops-in-drops microfluidics.Biomedical microdevices · 2016Article
- Retinal expression of small non-coding RNAs in a murine model of proliferative retinopathy.Scientific reports · 2016Article
- Exploration of the R code-based mathematical model for PMI estimation using profiling of RNA degradation in rat brain tissue at different temperatures.Forensic science, medicine, and pathology · 2015Article
- Identification of retinopathy of prematurity related miRNAs in hyperoxia-induced neonatal rats by deep sequencing.International journal of molecular sciences · 2014Article
- Article
- Reliable reference genes for normalization of gene expression in cucumber grown under different nitrogen nutrition.PloS one · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeOxygen-induced retinopathy (OIR) is a robust animal model of human retinopathy of prematurity that readily allows changes in retinal gene and microRNA (miRNA) expression in response to fluctuations in oxygen levels to be studied. We sought to identify small non-coding RNA (ncRNA) genes that showed stable expression upon exposure to varying levels of oxygen, with different developmental stages and in different rat strains, to act as reference genes for normalizing miRNA expression in a rat model of OIR.
methodsExpression of five small ncRNAs (U6 snRNA, miR-16, U87, 4.5S RNA (H) "Variant 1", and 5S ribosomal RNA [rRNA]) were tested on a standard RNA pool and representative retinal samples from P5, P6, P9, and P14 from room air- and cyclic hyperoxia-exposed rats using reverse transcription (RT)-qPCR, to assess the effect of developmental stage and exposure to fluctuations in oxygen levels, respectively. Two strains of inbred albino rats, Fischer 344 (F344, resistant to OIR) and Sprague-Dawley rats (SD, susceptible to OIR), were used to assess the effect of rat strain on the stability of the small ncRNAs.
resultsIn this rat model of OIR, 5S rRNA expression was variable with strain, fluctuations in oxygen levels, and developmental stage. U6 snRNA was stably expressed with changes in oxygen levels, and minimal variation was observed with strain and developmental stage. MiR-16 showed less stable expression with changes in oxygen levels and between strains compared to U6 snRNA. Some variation in expression in response to developmental stage was also observed. The PCR amplification efficiencies of the U6 snRNA and miR-16 TaqMan assays were 56% and 78%, respectively. U87 and 4.5S RNA (H) "Variant 1" expression varied with strain, exposure to cyclic hyperoxia, and in particular developmental stage, and was at low levels in the neonatal rat retina.
conclusionsWe conclude that U6 snRNA and miR-16 are the most suitable reference RNAs for normalizing miRNA expression, as they are relatively stable with strain, exposure to cyclic hyperoxia, and developmental stage in a rat model of OIR.
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