ArticleMolecular vision2013
Protective effect of caffeine against high sugar-induced transcription of microRNAs and consequent gene silencing: a study using lenses of galactosemic mice.
Article in Molecular vision, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Research progress on antioxidants and protein aggregation inhibitors in cataract prevention and therapy (Review).Molecular medicine reports · 2025Review
- The Epigenetic Effects of Coffee.Molecules (Basel, Switzerland) · 2023Review
- Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats.Toxicology research · 2019Article
- Effect of caffeine on biomarkers of oxidative stress in lenses of rats with streptozotocin-induced diabetes.Archives of medical science : AMS · 2019Article
- Caffeine induces sustained apoptosis of human gastric cancer cells by activating the caspase‑9/caspase‑3 signalling pathway.Molecular medicine reports · 2017Article
- MicroRNAs: new players in cataract.American journal of translational research · 2017Review
- Effect of coffee (caffeine) against human cataract blindness.Clinical ophthalmology (Auckland, N.Z.) · 2016Article
- High sugar-induced repression of antioxidant and anti-apoptotic genes in lens: reversal by pyruvate.Molecular and cellular biochemistry · 2015Article
- Protection from endotoxic uveitis by intravitreal Resolvin D1: involvement of lymphocytes, miRNAs, ubiquitin-proteasome, and M1/M2 macrophages.Mediators of inflammation · 2015Article
- MiRNA-26b inhibits the proliferation, migration, and epithelial-mesenchymal transition of lens epithelial cells.Molecular and cellular biochemistry · 2014Article
- MicroRNA associated with dyslipidemia and coronary disease in humans.Physiological genomics · 2013Article
- Sulforaphane-induced transcription of thioredoxin reductase in lens: possible significance against cataract formation.Clinical ophthalmology (Auckland, N.Z.) · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposePrevious studies have shown that caffeine prevents the formation of cataracts induced by a high-galactose diet and consequent oxidative stress. The objective of this study was to investigate if this protective effect is reflected in the attenuation of the transcription of microRNAs (miRNAs) known to induce apoptosis and cell death by gene silencing.
methodsYoung CD-1 mice were fed either a normal laboratory diet or a diet containing 25% galactose with or without 1% caffeine. One week later, the animals were euthanized, and the lenses isolated and promptly processed for RNA isolation and subsequent preparation of cDNAs by reverse transcriptase reaction. Mature miRNA (miR)-specific cDNAs were then quantified with PCR in a 96-well microRNA-specific cassette using an ABI7900HT PCR machine.
resultsAs expected from previous studies, the lenses were positive for all 84 miRs corresponding to the miRNA probes present in the cassette wells. However, the levels of at least 19 miRs were significantly elevated in galactosemic lenses compared to those in the normal lenses. The majority are proapoptotic. Such elevation was inhibited by caffeine. This has been demonstrated for the first time.
conclusionsSince aberrant elevation of miRNAs silences various genes and consequently deactivates protein translation, and since caffeine downregulates such aberration, the beneficial effect of caffeine could be attributed to its ability to suppress elevation of toxic miRs and consequent gene silencing.
Indexed as
Identifiers
23441122PMC3580991W176884152What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.