ArticleMolecular vision2013
Transplantation of tissue-engineered human corneal endothelium in cat models.
Article in Molecular vision, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- An Ex Vivo Morphometric Study of the Feline Corneal Endothelium (100 Eyes).Veterinary ophthalmology · 2026Article
- Animal Models in Eye Research: Focus on Corneal Pathologies.International journal of molecular sciences · 2023Review
- Biomaterials used for tissue engineering of barrier-forming cell monolayers in the eye.Frontiers in bioengineering and biotechnology · 2023Review
- Optimization of polycaprolactone - based nanofiber matrices for the cultivation of corneal endothelial cells.Scientific reports · 2021Article
- Article
- Prospects and Challenges of Translational Corneal Bioprinting.Bioengineering (Basel, Switzerland) · 2020Review
- Apoptotic effects of norfloxacin on corneal endothelial cells.Naunyn-Schmiedeberg's archives of pharmacology · 2020Article
- [Corneal cell therapy-an overview].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2017Review
- Engineered Basement Membranes for Regenerating the Corneal Endothelium.Advanced healthcare materials · 2016Article
- Cytotoxicity of atropine to human corneal endothelial cells by inducing mitochondrion-dependent apoptosis.Experimental biology and medicine (Maywood, N.J.) · 2016Article
- Substrates for Expansion of Corneal Endothelial Cells towards Bioengineering of Human Corneal Endothelium.Journal of functional biomaterials · 2015Review
- Corneal tissue engineering: recent advances and future perspectives.Tissue engineering. Part B, Reviews · 2015Review
- Clinical applications of naturally derived biopolymer-based scaffolds for regenerative medicine.Annals of biomedical engineering · 2015Review
- Use of Mesothelial Cells and Biological Matrices for Tissue Engineering of Simple Epithelium Surrogates.Frontiers in bioengineering and biotechnology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo evaluate the performance of reconstructed tissue-engineered human corneal endothelium (TE-HCE) by corneal transplantation in cat models.
methodsTE-HCE reconstruction was performed by culturing 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI)-labeled monoclonal HCE cells on denuded amniotic membranes (dAMs) in 20% fetal bovine serum-containing Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F12 (1:1) medium and 5% CO(2) at 37 ° C on a 24-well culture plate. The reconstructed TE-HCE was transplanted into cat corneas via lamellar keratoplasty with all of the endothelium and part of Descemet's membrane stripped. Postsurgical corneas were monitored daily with their histological properties examined during a period of 104 days after transplantation.
resultsThe reconstructed TE-HCE at a density of 3,413.33 ± 111.23 cells/mm(2) in average established intense cell-cell and cell-dAM junctions. After lamellar keratoplasty surgery, no obvious edema was found in TE-HCE-transplanted cat corneas, which were transparent throughout the monitoring period. In contrast, intense corneal edema developed in dAM-transplanted cat corneas, which were turbid. The corneal thickness gradually decreased to 751.33 ± 11.37 μm on day 104 after TE-HCE transplantation, while that of dAM eye was over 1,000 μm in thickness during the monitoring period. A monolayer of endothelium consisting of TE-HCE-originated cells at a density of 2,573.33 ± 0.59 cells/mm(2) attached tightly to the surface of remnant Descemet's membrane over 104 days; this was similar to the normal eye control in cell density.
conclusionsThe reconstructed TE-HCE was able to function as a corneal endothelium equivalent and restore corneal function in cat models.
Indexed as
Identifiers
23441111PMC3580986W1563196503What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.