Trial reportProceedings of the National Academy of Sciences of the United States of America2013
Effects of insufficient sleep on circadian rhythmicity and expression amplitude of the human blood transcriptome.
Trial report in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 285 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
285 citing papers in PubMed, 2 syntheses or guidelines pooled it, 572 citations in OpenAlex.
- Sleep Duration/Quality With Health Outcomes: An Umbrella Review of Meta-Analyses of Prospective Studies.Frontiers in medicine · 2021Pooled it
- Machine learning identifies a compact gene set for monitoring the circadian clock in human blood.Genome medicine · 2017Pooled it
- Efficacy and Safety ofNutrients · 2026Trial
- Short-term sleep restriction in humans alters diurnal circulating metabolite profiles, including those of microbial origin.The Journal of clinical investigation · 2026Trial
- Association between dim light melatonin onset predicted from gene expression profiles with sleep time and chronotype preference: A pilot study.Chronobiology international · 2025Trial
- An mHealth-Supported antenatal lifestyle intervention may be associated with improved maternal sleep in pregnancy: Secondary analysis from the PEARS trial.BJOG : an international journal of obstetrics and gynaecology · 2022Trial
- Vitamins DFrontiers in immunology · 2022Trial
- Response to therapeutic sleep deprivation: a naturalistic study of clinical and genetic factors and post-treatment depressive symptom trajectory.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2018Trial
- Neurobehavioral Impact of Successive Cycles of Sleep Restriction With and Without Naps in Adolescents.Sleep · 2017Trial
- Molecular correlates of sleep deprivation in the mouse brain identified by meta-analysis of microarray data.Neurobiology of sleep and circadian rhythms · 2026Article
- Article
- Explainable Machine Learning for Concurrent Screening of Elevated Work-Related Fatigue Using Routine Working-Hour and Occupational Health Data Among Manufacturing Workers: A Cross-Sectional Model Development and Internal Validation Study.Healthcare (Basel, Switzerland) · 2026Article
- Overnight Motor Memory Consolidation in Adolescents: Effects of Change in Dim Light Melatonin Onset After Sleep Restriction.Journal of biological rhythms · 2026Article
- Human data at odds and in confirmation of the two-process model of sleep regulation-a perspective.Npj biological timing and sleep · 2026Review
- D-Box Binding Protein (DBP) as a Circadian Output Regulator: Molecular Mechanisms, Tissue-Specific Functions, and Disease Relevance.International journal of molecular sciences · 2026Review
- Maternal sleep deprivation and developmental programming of brain aging trajectories in offspring.Biogerontology · 2026Review
- Integration of Transcriptomics With Interpretable Artificial Intelligence for Identifying Molecular Signatures of Physiological Stress in Sleep Deprivation.Journal of cellular and molecular medicine · 2026Article
- Transcriptional remodeling of human skeletal muscle following sleep restriction in postmenopausal women.Physiological genomics · 2026Article
- Diurnal Regulation and Gene-Specific Vulnerability of Oxidative Alcohol-Metabolizing Enzymes to Circadian Disruption.International journal of molecular sciences · 2026Article
- Catechins in insomnia-Alzheimer's disease comorbidity: A network pharmacology and molecular docking study.Medicine · 2026Article
225 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
Insufficient sleep and circadian rhythm disruption are associated with negative health outcomes, including obesity, cardiovascular disease, and cognitive impairment, but the mechanisms involved remain largely unexplored. Twenty-six participants were exposed to 1 wk of insufficient sleep (sleep-restriction condition 5.70 h, SEM = 0.03 sleep per 24 h) and 1 wk of sufficient sleep (control condition 8.50 h sleep, SEM = 0.11). Immediately following each condition, 10 whole-blood RNA samples were collected from each participant, while controlling for the effects of light, activity, and food, during a period of total sleep deprivation. Transcriptome analysis revealed that 711 genes were up- or down-regulated by insufficient sleep. Insufficient sleep also reduced the number of genes with a circadian expression profile from 1,855 to 1,481, reduced the circadian amplitude of these genes, and led to an increase in the number of genes that responded to subsequent total sleep deprivation from 122 to 856. Genes affected by insufficient sleep were associated with circadian rhythms (PER1, PER2, PER3, CRY2, CLOCK, NR1D1, NR1D2, RORA, DEC1, CSNK1E), sleep homeostasis (IL6, STAT3, KCNV2, CAMK2D), oxidative stress (PRDX2, PRDX5), and metabolism (SLC2A3, SLC2A5, GHRL, ABCA1). Biological processes affected included chromatin modification, gene-expression regulation, macromolecular metabolism, and inflammatory, immune and stress responses. Thus, insufficient sleep affects the human blood transcriptome, disrupts its circadian regulation, and intensifies the effects of acute total sleep deprivation. The identified biological processes may be involved with the negative effects of sleep loss on health, and highlight the interrelatedness of sleep homeostasis, circadian rhythmicity, and metabolism.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.