ArticleInfection and immunity2013
Virulence of the Shiga toxin type 2-expressing Escherichia coli O104:H4 German outbreak isolate in two animal models.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Broad time-dependent transcriptional activity of metabolic genes of E. coli O104:H4 strain C227/11Φcu in a soil microenvironment at low temperature.Environmental microbiology reports · 2023Article
- Sporadic Detection ofMicroorganisms · 2023Article
- Nasal immunization with H7 flagellin protects mice against hemolytic uremic syndrome secondary toFrontiers in cellular and infection microbiology · 2023Article
- Survival of EnterohemorrhagicPathogens (Basel, Switzerland) · 2021Article
- Human microbiota modulation via QseC sensor kinase mediated in the Escherichia coli O104:H4 outbreak strain infection in microbiome model.BMC microbiology · 2021Article
- A One Health Perspective for Defining and DecipheringComparative medicine · 2021Review
- Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-ProducingMicroorganisms · 2020Article
- Genetic and Virulence Profiles of EnteroaggregativeFrontiers in cellular and infection microbiology · 2020Article
- Differential transcriptome analysis of enterohemorrhagic Escherichia coli strains reveals differences in response to plant-derived compounds.BMC microbiology · 2019Article
- QseC Signaling in the Outbreak O104:H4Journal of bacteriology · 2019Article
- The Role of the AggR Regulon in the Virulence of the Shiga Toxin-Producing EnteroaggregativeFrontiers in microbiology · 2019Article
- Dextran Sulfate Sodium Colitis Facilitates Colonization with Shiga Toxin-Producing Escherichia coli: a Novel Murine Model for the Study of Shiga Toxicosis.Infection and immunity · 2018Article
- The wax moth Galleria mellonella as a novel model system to study Enteroaggregative Escherichia coli pathogenesis.Virulence · 2017Article
- EnteroaggregativemBio · 2017Article
- Turn Up the Heat-Food and ClinicalFrontiers in microbiology · 2017Article
- Changes in Gene Transcription Induced by Hydrogen Peroxide Treatment of Verotoxin-ProducingFrontiers in microbiology · 2017Article
- The primary transcriptome of the Escherichia coli O104:H4 pAA plasmid and novel insights into its virulence gene expression and regulation.Scientific reports · 2016Article
- Polysorbates prevent biofilm formation and pathogenesis of Escherichia coli O104:H4.Biofouling · 2016Article
- Virulence from vesicles: Novel mechanisms of host cell injury by Escherichia coli O104:H4 outbreak strain.Scientific reports · 2015Article
- Shiga toxin 2a and Enteroaggregative Escherichia coli--a deadly combination.Gut microbes · 2015Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
In May 2011, a large food-borne outbreak was traced to an unusual O104:H4 enteroaggregative Escherichia coli (EAEC) strain that produced Shiga toxin (Stx) type 2 (Stx2). We developed a mouse model to study the pathogenesis and treatment for this strain and examined the virulence of the isolate for Dutch belted rabbits. O104:H4 strain C227-11 was gavaged into C57BL/6 mice at 10(9) to 10(11) CFU/animal. The infected animals were then given water with ampicillin (Amp; 5 g/liter) ad libitum. The C227-11-infected, Amp-treated C57BL/6 mice exhibited both morbidity and mortality. Kidneys from mice infected with C227-11 showed acute tubular necrosis, a finding seen in mice infected with typical Stx-producing E. coli. We provided anti-Stx2 antibody after infection and found that all of the antibody-treated mice gained more weight than untreated mice and, in another study, that all of the antibody-treated animals lived, whereas 3/8 phosphate-buffered saline-treated mice died. We further compared the pathogenesis of C227-11 with that of an Stx-negative (Stx(-)) O104:H4 isolate, C734-09, and an Stx2(-) phage-cured derivative of C227-11. Whereas C227-11-infected animals lost weight or gained less weight over the course of infection and died, mice infected with either of the Stx(-) isolates did not lose weight and only one mouse died. When the Stx-positive (Stx(+)) and Stx2(-) O104:H4 strains were compared in rabbits, greater morbidity and mortality were observed in rabbits infected with the Stx2(+) isolates than the Stx2(-) isolates. In conclusion, we describe two animal models for EAEC pathogenesis, and these studies show that Stx2 is responsible for most of the virulence observed in C227-11-infected mice and rabbits.
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