ArticleThe American journal of tropical medicine and hygiene2013
Development of three PCR assays for the differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the co-endemic region of Qinghai-Tibet plateau, China.
Article in The American journal of tropical medicine and hygiene, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Spatiotemporal distribution of newly diagnosed echinococcosis patients in Sichuan Province, China, 2013-2024.PLoS neglected tropical diseases · 2025Article
- Delayed Diagnosis of Imported Cystic Echinococcosis and Successful Treatment With Percutaneous Drainage and Albendazole in Korea: A Case Report.Journal of Korean medical science · 2025Article
- Sensitive detection of specific cell-free DNA in serum samples from sheep with cystic echinococcosis.PLoS neglected tropical diseases · 2023Article
- Current status of cystic echinococcosis control in the Falkland Islands: has elimination been achieved?Parasitology · 2023Article
- IEg67 kDa Bovine Hydatid Cyst Antigen: A Candidate for Developing Sero-Diagnostic Assays for Cystic Echinococcosis, a Disease of One Health Importance.Animals : an open access journal from MDPI · 2023Article
- Echinococcus spp. and genotypes infecting humans in Tibet Autonomous Region of China: a molecular investigation with near-complete/complete mitochondrial sequences.Parasites & vectors · 2022Article
- Taeniid cestodes in Tibetan foxes (International journal for parasitology. Parasites and wildlife · 2020Article
- Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing.PLoS neglected tropical diseases · 2020Article
- Targeted Sequencing of Genomic Repeat Regions Detects Circulating Cell-free Echinococcus DNA.PLoS neglected tropical diseases · 2020Article
- Echinococcosis: Advances in the 21st Century.Clinical microbiology reviews · 2019Review
- Echinococcus multilocularis and Echinococcus shiquicus in a small mammal community on the eastern Tibetan Plateau: host species composition, molecular prevalence, and epidemiological implications.Parasites & vectors · 2018Article
- Development of LAMP assays for the molecular detection of taeniid infection in canine in Tibetan rural area.The Journal of veterinary medical science · 2017Article
- Latent class models for Echinococcus multilocularis diagnosis in foxes in Switzerland in the absence of a gold standard.Parasites & vectors · 2017Article
- A novel PCR-RFLP assay for molecular characterization of Echinococcus granulosus sensu lato and closely related species in developing countries.Parasitology research · 2016Article
- Discrimination between E. granulosus sensu stricto, E. multilocularis and E. shiquicus Using a Multiplex PCR Assay.PLoS neglected tropical diseases · 2015Article
- Independent evaluation of a canine Echinococcosis Control Programme in Hobukesar County, Xinjiang, China.Acta tropica · 2015Article
- Reinfection studies of canine echinococcosis and role of dogs in transmission of Echinococcus multilocularis in Tibetan communities, Sichuan, China.Parasitology · 2013Article
- Dog ownership, dog behaviour and transmission of Echinococcus spp. in the Alay Valley, southern Kyrgyzstan.Parasitology · 2013Article
- The Molecular Epidemiology of Cystic and Alveolar Echinococcosis in Southeast Turkey.Iranian journal of parasitologyArticle
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Authors and funding
8 authors.
Funding
Abstract
To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of Echinococcus shiquicus, Echinococcus granulosus G1, and Echinococcus multilocularis DNA derived from parasite tissue or canid fecal samples. Specificity using parasite tissue-derived DNA was found to be 100% except for E. shiquicus primers that faintly detected E. equinus DNA. Sensitivity of the three assays for DNA detection was between 2 and 10 pg. Ethanol precipitation of negative PCR fecal samples was used to eliminate false negatives and served to increase sensitivity as exemplified by an increase in detection from 0% to 89% of E. shiquicus coproDNA using necropsy-positive fox samples.
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