ReviewMolecular & cellular proteomics : MCP2013
Toward a platform for comprehensive glycan sequencing.
Review in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Article
- N-glycolylated carbohydrates in nature.Glycobiology · 2022Review
- An Integrative Glycomic Approach for Quantitative Meat Species Profiling.Foods (Basel, Switzerland) · 2022Article
- Mass Spectrometry-Based Techniques to Elucidate the Sugar Code.Chemical reviews · 2022Review
- Separation and Identification of Permethylated Glycan Isomers by Reversed Phase NanoLC-NSI-MSMolecular & cellular proteomics : MCP · 2021Article
- Dissection of Fragmentation Pathways in Protonated N-Acetylhexosamines.Analytical chemistry · 2018Article
- Building a PGC-LC-MS N-glycan retention library and elution mapping resource.Glycoconjugate journal · 2018Article
- Advancing a High Throughput Glycotope-centric Glycomics Workflow Based on nanoLC-MSMolecular & cellular proteomics : MCP · 2017Article
- Simultaneous quantification of N- and O-glycans using a solid-phase method.Nature protocols · 2017Article
- LC-MS/MS analysis of permethylated N-glycans facilitating isomeric characterization.Analytical and bioanalytical chemistry · 2017Article
- Simultaneous analyses of N-linked and O-linked glycans of ovarian cancer cells using solid-phase chemoenzymatic method.Clinical proteomics · 2017Article
- Lactation and Intestinal Microbiota: How Early Diet Shapes the Infant Gut.Journal of mammary gland biology and neoplasia · 2015Review
- Benchmark study of automatic annotation of MALDI-TOF N-glycan profiles.Journal of proteomics · 2015Article
- Characteristic Changes in Cell Surface Glycosylation Accompany Intestinal Epithelial Cell (IEC) Differentiation: High Mannose Structures Dominate the Cell Surface Glycome of Undifferentiated Enterocytes.Molecular & cellular proteomics : MCP · 2015Article
- Targeted methods for quantitative analysis of protein glycosylation.Proteomics. Clinical applications · 2015Review
- Tools to MSn Sequence and Document the Structures of Glycan Epitopes.Discovering the subtleties of sugars : proceedings of the 3rd Beilstein Glyco-Bioinformatics Symposium : June 10th - 14th, 2013, Potsdam, Germany. International Beilstein Symposium on Glyco-Bioinformatics (3rd : 2013 : Potsdam, Germany) · 2014Article
- Structural documentation of glycan epitopes: sequential mass spectrometry and spectral matching.Journal of the American Society for Mass Spectrometry · 2014Article
- Oligosaccharide analysis by mass spectrometry: a review of recent developments.Analytical chemistry · 2014Review
- Breast milk oligosaccharides: structure-function relationships in the neonate.Annual review of nutrition · 2014Review
- Glycoproteomic and glycomic databases.Clinical proteomics · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
From a series of recently published reports, an analytical platform has been proposed for a quantitative and qualitative measure of N- and O-glycosylation, complete with peptide-glycan connectivity and detailed structural understanding. As distant as this may appear, a best methods approach will appear that must move us beyond the cartoon stage of structural understanding. Thus, with this unifying goal in mind, we summarize a series of individually promising first phase protocols of sample preparation (release, purification, and quantification) that remain congruent with a concluding phase (methylation and MS(n)) for documented structural detail. Sequential enzymatic N-glycan and chemical O-glycan release from glycopeptides with intervening solid phase extraction and derivatization will provide for a comparative quantification measure of glycosylation. The O-glycan release will be nonreductive and coupled with Michael addition to a pyrazolone analog (1-phenyl-3-methyl-5-pyrazolone) with both the peptide and glycan labeled. The product glycans are stable to methylation and appropriate for sequential disassembly (MS(n)). An application using human serum and cancer samples has been detailed characterizing sLe(x) and comparable valence epitopes. This integrated platform will provide opportunities at variable points to contrast, share, and advance alternative protocols in a collaborative effort that is greatly needed. This integrated platform provides end point opportunities to confirm structural details compiled from synthetic standards and well characterized biologics by MS(n).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.