ArticleMolecular and cellular biology2013
A weak spliceosome-binding domain of Yju2 functions in the first step and bypasses Prp16 in the second step of splicing.
Article in Molecular and cellular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- New mechanistic insights into Prp22-mediated exon ligation and mRNA release.Nucleic acids research · 2025Article
- Article
- Control of 3' splice site selection by the yeast splicing factor Fyv6.bioRxiv : the preprint server for biology · 2024Article
- Characterization of Cwc2, U6 snRNA, and Prp8 interactions destabilized by Prp16 ATPase at the transition between the first and second steps of splicing.RNA (New York, N.Y.) · 2024Article
- Spliceosome assembly and regulation: insights from analysis of highly reduced spliceosomes.RNA (New York, N.Y.) · 2023Review
- Structural basis for conformational equilibrium of the catalytic spliceosome.Molecular cell · 2021Article
- Postcatalytic spliceosome structure reveals mechanism of 3'-splice site selection.Science (New York, N.Y.) · 2017Article
- A genetic screen implicates a CWC16/Yju2/CCDC130 protein and SMU1 in alternative splicing inRNA (New York, N.Y.) · 2017Article
- A close-up look at the spliceosome, at last.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A central role of Cwc25 in spliceosome dynamics during the catalytic phase of pre-mRNA splicing.RNA (New York, N.Y.) · 2017Article
- Role of Cwc24 in the First Catalytic Step of Splicing and Fidelity of 5' Splice Site Selection.Molecular and cellular biology · 2017Article
- Dramatically reduced spliceosome in Cyanidioschyzon merolae.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence.Genes & development · 2015Article
- Structural requirement of Ntc77 for spliceosome activation and first catalytic step.Nucleic acids research · 2014Article
- Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.Eukaryotic cell · 2013Article
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2 authors.
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Abstract
Yju2 is an essential splicing factor required for the first catalytic step after the action of Prp2. We dissected the structure of Yju2 and found that the amino (Yju2-N) and carboxyl (Yju2-C) halves of the protein can be separated and reconstituted for Yju2 function both in vivo and in vitro. Yju2-N has a weak affinity for the spliceosome but functions in promoting the first reaction, with the second reaction being severely impeded. The association of Yju2-N with the spliceosome is stabilized by the presence of Yju2-C at both the precatalytic and postcatalytic stages. Strikingly, Yju2-N supported a low level of the second reaction even in the absence of Prp16. Prp16 is known to mediate destabilization of Yju2 and Cwc25 after the first reaction to allow progression of the second reaction. We propose that in the absence of the C domain, Yju2-N is not stably associated with the spliceosome after lariat formation, and thus bypasses the need for Prp16. We also showed, by UV cross-linking, that Yju2 directly contacts U2 snRNA primarily in the helix II region both pre- and postcatalytically and in the branch-binding region only at the precatalytic stage, suggesting a possible role for Yju2 in positioning the branch point during the first reaction.
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