ArticleNitric oxide : biology and chemistry2013
Proteomic analysis of the NOS2 interactome in human airway epithelial cells.
Article in Nitric oxide : biology and chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- The sequenced rat brain transcriptome--its use in identifying networks predisposing alcohol consumption.The FEBS journal · 2015Pooled it
- Regulation of Inducible Nitric Oxide Synthase (NOS2) Expression in Healthy and Inflamed Bowel: A Narrative Review.International journal of molecular sciences · 2026Review
- PGE2 regulates ferroptosis and osteogenesis of MC3T3-E1 cells via NOS2.Scientific reports · 2026Article
- Comparison of the Nitric Oxide Synthase Interactomes and S-Nitroso-Proteomes: Furthering the Case for Enzymatic S-Nitrosylation.Antioxidants & redox signaling · 2023Article
- A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types.Cell communication and signaling : CCS · 2022Article
- FBXO45 is a potential therapeutic target for cancer therapy.Cell death discovery · 2020Review
- Relationship between Particulate Matter (PMCanadian respiratory journal · 2020Article
- Gasotransmitters in pregnancy: from conception to uterine involution.Biology of reproduction · 2019Review
- Molecular Mechanisms of Nitric Oxide in Cancer Progression, Signal Transduction, and Metabolism.Antioxidants & redox signaling · 2019Review
- Regulation of mycobacterial infection by macrophage Gch1 and tetrahydrobiopterin.Nature communications · 2018Article
- A Novel Glycoproteomics Workflow Reveals Dynamic O-GlcNAcylation of COPγ1 as a Candidate Regulator of Protein Trafficking.Frontiers in endocrinology · 2018Article
- Signaling and stress: The redox landscape in NOS2 biology.Free radical biology & medicine · 2015Review
- Atypical ubiquitin E3 ligase complex Skp1-Pam-Fbxo45 controls the core epithelial-to-mesenchymal transition-inducing transcription factors.Oncotarget · 2015Article
- Proteomic response to 5,6-dimethylxanthenone 4-acetic acid (DMXAA, vadimezan) in human non-small cell lung cancer A549 cells determined by the stable-isotope labeling by amino acids in cell culture (SILAC) approach.Drug design, development and therapy · 2015Article
- Fbxo45 inhibits calcium-sensitive proteolysis of N-cadherin and promotes neuronal differentiation.The Journal of biological chemistry · 2014Article
- A substrate trapping approach identifies proteins regulated by reversible S-nitrosylation.Molecular & cellular proteomics : MCP · 2014Article
- Genetic predictors associated with improvement of asthma symptoms in response to inhaled corticosteroids.The Journal of allergy and clinical immunology · 2014Article
- Evolution of glucose utilization: glucokinase and glucokinase regulator protein.Molecular phylogenetics and evolution · 2014Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
The cytokine-inducible isoform of nitric oxide synthase (NOS2) is constitutively expressed in human respiratory epithelia and is upregulated in inflammatory lung disease. Here, we sought to better define the protein interactions that may be important for NOS2 activity and stability, as well as to identify potential targets of NOS2-derived NO, in the respiratory epithelium. We overexpressed Flag-tagged, catalytically-inactive NOS2 in A549 cells and used mass spectrometry to qualitatively identify NOS2 co-immunoprecipitating proteins. Stable isotope labeling of amino acids in cell culture (SILAC) was used to quantify the coordinate effects of cytokine stimulation on NOS2-protein interactions. Multi-protein networks dominated the NOS2 interactome, and cytokine-inducible interactions with allosteric activators and with the ubiquitin-proteasome system were correlated with cytokine-dependent increases in NO metabolites and in NOS2 ubiquitination. The ubiquitin ligase scaffolding protein, FBXO45, was identified as a novel, direct NOS2 interactor. Similar to the SPRY domain-containing SOCS box (SPSB) proteins, FBXO45 requires Asn27 in the (23)DINNN(27) motif of NOS2 for its interaction. However, FBXO45 is unique from the SPSBs in that it recruits a distinct E3 ligase complex containing MYCBP2 and SKP1. Collectively, these findings demonstrate the general utility of interaction proteomics for defining new aspects of NOS2 physiology.
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Registered trials
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