ArticleThe journal of sexual medicine2013
Effect of estrogen on molecular and functional characteristics of the rodent vaginal muscularis.
Article in The journal of sexual medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.
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Who cites it
8 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Animal models for pelvic organ prolapse: systematic review.International urogynecology journal · 2021Pooled it
- Vaginal biomechanical function in premenopausal and postmenopausal women with and without pelvic organ prolapse.Scientific reports · 2025Article
- Assessment of the Elastographic and Electromyographic of Pelvic Floor Muscles in Postmenopausal Women with Stress Urinary Incontinence Symptoms.Diagnostics (Basel, Switzerland) · 2021Article
- Contractile Properties of Vaginal Tissue.Journal of biomechanical engineering · 2020Review
- Tissue Composition and Biomechanical Property Changes in the Vaginal Wall of Ovariectomized Young Rats.BioMed research international · 2019Article
- Effects ofBehavioural neurology · 2018Article
- Relationship between lower limb position and pelvic floor muscle surface electromyography activity in menopausal women: a prospective observational study.Clinical interventions in aging · 2017Observational
- The standardized BHH10 extract, a combination of Astragalus membranaceus, Cinnamomum cassia, and Phellodendron amurense, reverses bone mass and metabolism in a rat model of postmenopausal osteoporosis.Phytotherapy research : PTR · 2015Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
introductionVaginal atrophy is a consequence of menopause; however, little is known concerning the effect of a decrease in systemic estrogen on vaginal smooth muscle structure and function. As the incidence of pelvic floor disorders increases with age, it is important to determine if estrogen regulates the molecular composition and contractility of the vaginal muscularis.
aimThe goal of this study was to determine the effect of estrogen on molecular and functional characteristics of the vaginal muscularis utilizing a rodent model of surgical menopause.
methodsThree- to 4-month old Sprague-Dawley rats underwent sham laparotomy (Sham, N = 18) or ovariectomy (Ovx, N = 39). Two weeks following surgery, animals received a subcutaneous osmotic pump containing vehicle (Sham, Ovx) or 17β-estradiol (Ovx). Animals were euthanized 1 week later, and the proximal vagina was collected for analysis of contractile protein expression and in vitro studies of contractility. Measurements were analyzed using a one-way analysis of variance followed by Tukey's post hoc analysis (α = 0.05).
main outcome measuresProtein and mRNA transcript expression levels of contractile proteins, in vitro measurements of vaginal contractility.
resultsOvariectomy decreased the expression of carboxyl-terminal myosin heavy chain isoform (SM1) and h-caldesmon and reduced the amplitude of contraction of the vaginal muscularis in response to KCl. Estradiol replacement reversed these changes. No differences were detected in the % vaginal muscularis, mRNA transcript expression of amino-terminal MHC isoforms, l-caldesmon expression, and maximal velocity of shortening.
conclusionSystemic estrogen replacement restores functional and molecular characteristics of the vaginal muscularis of ovariectomized rats. Our results indicate that menopause is associated with changes in the vaginal muscularis, which may contribute to the increased incidence of pelvic floor disorders with age.
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