ArticlePloS one2013
Enhancing post-expansion chondrogenic potential of costochondral cells in self-assembled neocartilage.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 33 citations in OpenAlex.
- Cartilage structure-inspired nanofiber-hydrogel composite with robust proliferation and stable chondral lineage-specific differentiation function to orchestrate cartilage regeneration for artificial tracheal construction.Bioactive materials · 2025Article
- Proteomic, mechanical, and biochemical development of tissue-engineered neocartilage.Biomaterials research · 2022Article
- The Effect of Neonatal, Juvenile, and Adult Donors on Rejuvenated Neocartilage Functional Properties.Tissue engineering. Part A · 2022Article
- The functionality and translatability of neocartilage constructs are improved with the combination of fluid-induced shear stress and bioactive factors.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Remaining Hurdles for Tissue-Engineering the Temporomandibular Joint Disc.Trends in molecular medicine · 2019Review
- Autologous costal chondral transplantation and costa-derived chondrocyte implantation: emerging surgical techniques.Therapeutic advances in musculoskeletal disease · 2019Review
- Considerations for translation of tissue engineered fibrocartilage from bench to bedside.Journal of biomechanical engineering · 2018Article
- Overcoming Challenges in Engineering Large, Scaffold-Free Neocartilage with Functional Properties.Tissue engineering. Part A · 2018Article
- A Comparison of Bone Marrow and Cord Blood Mesenchymal Stem Cells for Cartilage Self-Assembly.Tissue engineering. Part A · 2018Article
- Tissue engineering toward temporomandibular joint disc regeneration.Science translational medicine · 2018Article
- Characterization of costal cartilage and its suitability as a cell source for articular cartilage tissue engineering.Journal of tissue engineering and regenerative medicine · 2018Article
- Using Costal Chondrocytes to Engineer Articular Cartilage with Applications of Passive Axial Compression and Bioactive Stimuli.Tissue engineering. Part A · 2018Article
- Effects of passage number and post-expansion aggregate culture on tissue engineered, self-assembled neocartilage.Acta biomaterialia · 2016Article
- Cell-based tissue engineering strategies used in the clinical repair of articular cartilage.Biomaterials · 2016Review
- Superficial Zone Extracellular Matrix Extracts Enhance Boundary Lubrication of Self-Assembled Articular Cartilage.Cartilage · 2016Article
- Articular cartilage tissue engineering: the role of signaling molecules.Cellular and molecular life sciences : CMLS · 2016Review
- A preclinical evaluation of an autologous living hyaline-like cartilaginous graft for articular cartilage repair: a pilot study.Scientific reports · 2015Article
- Neocartilage integration in temporomandibular joint discs: physical and enzymatic methods.Journal of the Royal Society, Interface · 2015Article
- Engineering a fibrocartilage spectrum through modulation of aggregate redifferentiation.Cell transplantation · 2015Article
- Surface zone articular chondrocytes modulate the bulk and surface mechanical properties of the tissue-engineered cartilage.Tissue engineering. Part A · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The insufficient healing capacity of articular cartilage necessitates mechanically functional biologic tissue replacements. Using cells to form biomimetic cartilage implants is met with the challenges of cell scarcity and donor site morbidity, requiring expanded cells that possess the ability to generate robust neocartilage. To address this, this study assesses the effects of expansion medium supplementation (bFGF, TFP, FBS) and self-assembled construct seeding density (2, 3, 4 million cells/5 mm dia. construct) on the ability of costochondral cells to generate biochemically and biomechanically robust neocartilage. Results show TFP (1 ng/mL TGF-β1, 5 ng/mL bFGF, 10 ng/mL PDGF) supplementation of serum-free chondrogenic expansion medium enhances the post-expansion chondrogenic potential of costochondral cells, evidenced by increased glycosaminoglycan content, decreased type I/II collagen ratio, and enhanced compressive properties. Low density (2 million cells/construct) enhances matrix synthesis and tensile and compressive mechanical properties. Combined, TFP and Low density interact to further enhance construct properties. That is, with TFP, Low density increases type II collagen content by over 100%, tensile stiffness by over 300%, and compressive moduli by over 140%, compared with High density. In conclusion, the interaction of TFP and Low density seeding enhances construct material properties, allowing for a mechanically functional, biomimetic cartilage to be formed using clinically relevant costochondral cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.