ArticlePloS one2013
A widespread and unusual RNA trans-splicing type in dinoflagellate mitochondria.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- The complete mitochondrial genome ofFrontiers in microbiology · 2022Article
- The Elusive Mitochondrial Genomes of Apicomplexa: Where Are We Now?Frontiers in microbiology · 2021Review
- Single-Cell Transcriptomics of Abedinium Reveals a New Early-Branching Dinoflagellate Lineage.Genome biology and evolution · 2020Article
- Keeping it complicated: Mitochondrial genome plasticity across diplonemids.Scientific reports · 2017Article
- Peculiarities within peculiarities - dinoflagellates and their mitochondrial genomes.Mitochondrial DNA. Part B, Resources · 2017Article
- Major transitions in dinoflagellate evolution unveiled by phylotranscriptomics.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Group II Intron-Mediated Trans-Splicing in the Gene-Rich Mitochondrial Genome of an Enigmatic Eukaryote, Diphylleia rotans.Genome biology and evolution · 2016Article
- The Large Mitochondrial Genome of Symbiodinium minutum Reveals Conserved Noncoding Sequences between Dinoflagellates and Apicomplexans.Genome biology and evolution · 2015Article
- Programmed Genome Rearrangements in the Ciliate Oxytricha.Microbiology spectrum · 2014Review
- A large number of nuclear genes in the human parasite blastocystis require mRNA polyadenylation to create functional termination codons.Genome biology and evolution · 2014Article
- Transcription and Maturation of mRNA in Dinoflagellates.Microorganisms · 2013Review
- Mitochondrial Genes of Dinoflagellates Are Transcribed by a Nuclear-Encoded Single-Subunit RNA Polymerase.PloS one · 2013Article
- Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytochrome oxidase subunit 3 (Cox3) is a mitochondrion-encoded core membrane protein of complex IV of the mitochondrial respiratory chain, and consists of seven trans-membrane helices. Here we show that in diverse later-branching dinoflagellates, cox3 is consistently split into two exons in the mitochondrial genome between helices six and seven. Gene exons are transcribed as two discrete oligoadenylated precursor RNAs, and these are subsequently trans-spliced to form a complete coding mRNA. This trans-splicing is highly unusual in that some of the oligoadenylated tail is incorporated at the splice site, such that a short string of adenosines links the two coding exons. This feature is consistently represented in diverse dinoflagellates, however the number of adenosines added varies according to the size of the coding gap between the two exons. Thus we observed between zero (Amphidinium carterae) and 10 (Symbiodinium sp.) adenosines added in different taxa, but the final coding sequence length is identical with the reading frame maintained. Northern analyses show that precursor cox3 transcripts are approximately equally abundant as mature cox3 mRNAs, suggesting a slow or regulated maturation process. These data indicate that the splicing mechanism in dinoflagellate mitochondria is tolerant of variations in the length of the precursor coding sequence, and implicates the use of a splicing template, or guide molecule, during splicing that controls mature mRNA length.
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