ArticlePloS one2013
Polycomb domain formation depends on short and long distance regulatory cues.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Unraveling the complexity of the histone code: implications for gene regulation and disease.Genome biology · 2026Review
- Runx2 downregulates Lpl expression through super-silencer formation to alter lipid metabolism in Zhu Schwann cells after nerve injury.Cellular & molecular biology letters · 2025Article
- Determining the functional relationship between epigenetic and physical chromatin domains in Drosophila.Genome biology · 2025Article
- Long-Distance Repression by Human Silencers: Chromatin Interactions and Phase Separation in Silencers.Cells · 2022Review
- Integrated bioinformatics analysis reveals that EZH2-rich domains promote transcriptional repression in cervical cancer.EXCLI journal · 2022Article
- H3K27me3-rich genomic regions can function as silencers to repress gene expression via chromatin interactions.Nature communications · 2021Article
- Defining the Boundaries of Polycomb Domains inGenetics · 2020Article
- Separate Polycomb Response Elements control chromatin state and activation of the vestigial gene.PLoS genetics · 2019Article
- The Ubx Polycomb response element bypasses an unpaired Fab-8 insulator via cis transvection in Drosophila.PloS one · 2018Article
- Generalized nucleation and looping model for epigenetic memory of histone modifications.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Spectral identification of topological domains.Bioinformatics (Oxford, England) · 2016Article
- Formation of a Polycomb-Domain in the Absence of Strong Polycomb Response Elements.PLoS genetics · 2016Article
- Review
- The Arabidopsis GAGA-Binding Factor BASIC PENTACYSTEINE6 Recruits the POLYCOMB-REPRESSIVE COMPLEX1 Component LIKE HETEROCHROMATIN PROTEIN1 to GAGA DNA Motifs.Plant physiology · 2015Article
- A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila.Epigenetics & chromatin · 2015Article
- Expanding the roles of chromatin insulators in nuclear architecture, chromatin organization and genome function.Cellular and molecular life sciences : CMLS · 2014Review
- Insulator function and topological domain border strength scale with architectural protein occupancy.Genome biology · 2014Article
- Are we there yet? Initial targeting of the Male-Specific Lethal and Polycomb group chromatin complexes in Drosophila.Open biology · 2014Review
- Surviving an identity crisis: a revised view of chromatin insulators in the genomics era.Biochimica et biophysica acta · 2014Review
- YY1 DNA binding and interaction with YAF2 is essential for Polycomb recruitment.Nucleic acids research · 2014Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPolycomb group (PcG) proteins dynamically define cellular identities through the epigenetic repression of key developmental genes. In Drosophila, cis-regulatory regions termed PcG response elements (PREs) act as nucleation sites for PcG proteins to create large repressive PcG domains that are marked by trimethylation of lysine 27 on histone H3 (H3K27me3). In addition to an action in cis, PREs can interact over long distances, thereby enhancing PcG dependent silencing. How PcG domains are established, which factors limit their propagation in cis, and how long range interactions of PREs in trans affect the chromatin structure is largely unknown. PRINCIPAL
findingsWe demonstrate that the insertion of a PRE-containing transgene in the Drosophila genome generates an artificial PcG domain and we analyze its organization by quantitative ChIP and ChIP-on-chip experiments. Intriguingly, a boundary element and known insulator proteins do not necessarily interfere with spreading of H3K27me3. Instead, domain borders correlate with the presence of promoter regions bound by RNA Polymerase II and active chromatin marks. In contrast, genes that are silent during early fly development get included within the PcG domain and this incorporation interferes with gene activation at later developmental stages. Moreover, trans-interaction of the transgenic PRE with its homologous endogenous PRE results in increased PcG binding, correlating with reinforced silencing of genes within the domain borders.
conclusionsOur results suggest that higher-order organization of PcG-bound chromatin can stabilize gene silencing within PcG domains. Further we propose that multi-protein complexes associated with active promoters are able to define the limits of PcG domains. Future work aimed to pinpoint the factors providing this barrier function will be required to understand the precise molecular mechanism by which active promoter regions can act as boundaries to stop spreading of H3K27me3.
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