Evidence map›Paper›PMID 23437125›Full record

ArticlePloS one2013

The Drosophila splicing factor PSI is phosphorylated by casein kinase II and tousled-like kinase.

J Matthew Taliaferro, Dhruv Marwha, Julie L Aspden, Daniela Mavrici, Nathalie E Cheng, Lori A Kohlstaedt, Donald C Rio

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

J Matthew TaliaferroDepartment of Molecular and Cell Biology, University of California, Berkeley, California, USA.
Dhruv Marwha
Julie L Aspden
Daniela Mavrici
Nathalie E Cheng
Lori A Kohlstaedt
Donald C Rio

Funding

BIOCHEMISTRY OF REGULATED PRE-MRNA SPLICING/DROSOPHILAR01GM061987 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI RIO, DONALD C · 2000 to 2010
$3.0M
Alternative pre-mRNA Splicing in DrosophilaR01GM097352 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI RIO, DONALD C · 2012 to 2015
$1.6M
Human THAP9-an active DNA transposaseR01GM104385 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI RIO, DONALD C · 2013 to 2016
$1.4M
Methods for purification of individual nuclear pre-messenger RNA-protein complexeR01GM094890 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI RIO, DONALD C · 2010 to 2013
$1.2M
LTQ XL Linear Quadrupole Ion Trap Mass SpectrometerS10RR025622 · NCRR · UNIVERSITY OF CALIFORNIA BERKELEY · PI KOHLSTAEDT, LORI · 2009 to 2009
$463k
NCRR NIH HHS S10 RR025622NCRR NIH HHS S10RR025622NIGMS NIH HHS GM094890-01NIGMS NIH HHS GM097352-01A1NIGMS NIH HHS GM61987NIGMS NIH HHS R01 GM061987NIGMS NIH HHS R01 GM094890NIGMS NIH HHS R01 GM097352NIGMS NIH HHS R01 GM104385
6 · The paper itself

Abstract

Alternative splicing of pre-mRNA is a highly regulated process that allows cells to change their genetic informational output. These changes are mediated by protein factors that directly bind specific pre-mRNA sequences. Although much is known about how these splicing factors regulate pre-mRNA splicing events, comparatively little is known about the regulation of the splicing factors themselves. Here, we show that the Drosophila splicing factor P element Somatic Inhibitor (PSI) is phosphorylated at at least two different sites by at minimum two different kinases, casein kinase II (CK II) and tousled-like kinase (tlk). These phosphorylation events may be important for regulating protein-protein interactions involving PSI. Additionally, we show that PSI interacts with several proteins in Drosophila S2 tissue culture cells, the majority of which are splicing factors.

Indexed as

AnimalsCasein Kinase IIDrosophila melanogasterDrosophila ProteinsMass SpectrometryMutationNuclear ProteinsPhosphorylationProtein Interaction MappingProtein Serine-Threonine KinasesRNA-Binding ProteinsRNA SplicingCasein Kinase IIDrosophila ProteinsNuclear ProteinsProtein Serine-Threonine KinasesPSI protein, DrosophilaRNA-Binding Proteinstlk protein, Drosophila

Identifiers

PMID23437125
PMCPMC3577899

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.