ArticlePloS one2013
Nanostructured 3D constructs based on chitosan and chondroitin sulphate multilayers for cartilage tissue engineering.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 131 citations in OpenAlex.
- Influence of Chondroitin Sulfate Concentration on the Physicochemical Properties of Acetaminophen-Releasing Cellulose/PVOH Membranes.ACS omega · 2026Article
- Recent Developments in Layer-by-Layer Assembly for Drug Delivery and Tissue Engineering Applications.Advanced healthcare materials · 2024Review
- Application of three-dimensional cell culture technology in screening anticancer drugs.Biotechnology letters · 2023Review
- Chitosan-Based Biomaterials: Insights into Chemistry, Properties, Devices, and Their Biomedical Applications.Marine drugs · 2023Review
- LbL Nano-Assemblies: A Versatile Tool for Biomedical and Healthcare Applications.Nanomaterials (Basel, Switzerland) · 2022Review
- Fabrication andCell journal · 2022Article
- Biopolymer-Based Multilayer Capsules and Beads Made via Templating: Advantages, Hurdles and Perspectives.Nanomaterials (Basel, Switzerland) · 2021Review
- Polyelectrolyte Multilayer Capsule (PEMC)-Based Scaffolds for Tissue Engineering.Micromachines · 2020Review
- Bioinspired multilayer membranes as potential adhesive patches for skin wound healing.Biomaterials science · 2018Article
- Biomimetic Layer-by-Layer Self-Assembly of Nanofilms, Nanocoatings, and 3D Scaffolds for Tissue Engineering.International journal of molecular sciences · 2018Review
- Sub-micrometer Sized, 3D-Surface-attached Polymer Networks by Microcontact Printing: Using UV-Crosslinking Efficiency to Tune Structure Height.Macromolecules · 2018Article
- Regenerative Engineering for Knee Osteoarthritis Treatment: Biomaterials and Cell-Based Technologies.Engineering (Beijing, China) · 2017Article
- Engineering Cell Surfaces with Polyelectrolyte Materials for Translational Applications.Polymers · 2017Review
- Encapsulation of human elastic cartilage-derived chondrocytes in nanostructured fibrin-agarose hydrogels.Histochemistry and cell biology · 2017Article
- Biomimetic microbeads containing a chondroitin sulfate/chitosan polyelectrolyte complex for cell-based cartilage therapy.Journal of materials chemistry. B · 2015Article
- Three dimensional de novo micro bone marrow and its versatile application in drug screening and regenerative medicine.Experimental biology and medicine (Maywood, N.J.) · 2015Review
- Silver-containing antimicrobial membrane based on chitosan-TPP hydrogel for the treatment of wounds.Journal of materials science. Materials in medicine · 2015Article
- Chitosan and Its Potential Use as a Scaffold for Tissue Engineering in Regenerative Medicine.BioMed research international · 2015Review
- Photocrosslinked ultrathin anionic polysaccharide supports for accelerated growth of human mesenchymal stem cells.Cell proliferation · 2014Article
- Tailored freestanding multilayered membranes based on chitosan and alginate.Biomacromolecules · 2014Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanostructured three-dimensional constructs combining layer-by-layer technology (LbL) and template leaching were processed and evaluated as possible support structures for cartilage tissue engineering. Multilayered constructs were formed by depositing the polyelectrolytes chitosan (CHT) and chondroitin sulphate (CS) on either bidimensional glass surfaces or 3D packet of paraffin spheres. 2D CHT/CS multi-layered constructs proved to support the attachment and proliferation of bovine chondrocytes (BCH). The technology was transposed to 3D level and CHT/CS multi-layered hierarchical scaffolds were retrieved after paraffin leaching. The obtained nanostructured 3D constructs had a high porosity and water uptake capacity of about 300%. Dynamical mechanical analysis (DMA) showed the viscoelastic nature of the scaffolds. Cellular tests were performed with the culture of BCH and multipotent bone marrow derived stromal cells (hMSCs) up to 21 days in chondrogenic differentiation media. Together with scanning electronic microscopy analysis, viability tests and DNA quantification, our results clearly showed that cells attached, proliferated and were metabolically active over the entire scaffold. Cartilaginous extracellular matrix (ECM) formation was further assessed and results showed that GAG secretion occurred indicating the maintenance of the chondrogenic phenotype and the chondrogenic differentiation of hMSCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.