ArticlePloS one2013
Detection of genetic diversity in Campylobacter jejuni isolated from a commercial turkey flock using flaA typing, MLST analysis and microarray assay.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Antimicrobial resistance, genetic diversity and virulence associated factors ofFrontiers in veterinary science · 2026Article
- Comparative Genomic Analysis of Livestock-DerivedJournal of microbiology and biotechnology · 2025Article
- SVR-flaA typing of erythromycin- and ciprofloxacin-resistant Campylobacter jejuni strains isolated from poultry slaughterhouses in southern Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Genomic diversity and antimicrobial resistance of Campylobacter spp. from humans and livestock in Nigeria.Journal of biomedical science · 2022Article
- Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Bacteria in Trinidad and Tobago.International journal of microbiology · 2021Review
- Antimicrobial Resistance Profiles, Virulence Genes, and Genetic Diversity of ThermophilicFrontiers in microbiology · 2021Article
- Molecular determination of genetic diversity amongInfection ecology & epidemiology · 2020Article
- Comparative restriction enzyme mapping of Campylobacter jejuni isolates from turkeys and broilers based on flaA flagellar gene using HpyF3I endonuclease.Folia microbiologica · 2019Article
- Genetic Diversity ofFrontiers in microbiology · 2019Article
- Genomic Analysis of Chilean Strains ofBioMed research international · 2019Article
- Typing ofVeterinary and animal science · 2018Article
- Antimicrobial resistance inGut pathogens · 2018Article
- Dairy Cattle, a Potential Reservoir of Human Campylobacteriosis: Epidemiological and Molecular Characterization ofFrontiers in microbiology · 2018Article
- Inquiring into the Gaps of Campylobacter Surveillance Methods.Veterinary sciences · 2017Review
- Prevalence, genotyping and risk factors of thermophilic Campylobacter spreading in organic turkey farms in Germany.Gut pathogens · 2016Article
- Genotyping and antibiotic resistance of thermophilic Campylobacter isolated from chicken and pig meat in Vietnam.Gut pathogens · 2016Article
- Genetic Characterization of Campylobacter Jejuni and C. coli Isolated From Broilers Using flaA PCR-Restriction Fragment Length Polymorphism Method in Shiraz, Southern Iran.Jundishapur journal of microbiology · 2015Article
- Colonisation of a phage susceptible Campylobacter jejuni population in two phage positive broiler flocks.PloS one · 2014Article
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Campylobacter is genetically highly diverse and undergoes frequent intraspecific recombination. Turkeys have been identified as an important reservoir for Campylobacter jejuni which is of public health significance. The assessment of the genetic diversity among Campylobacter population is critical for our understanding of the epidemiology of this bacterium. The genetic profiles were different according to the molecular typing methods used. The performance of established flaA genotyping, multilocus sequencing typing (MLST) and DNA microarray assay based on the ArrayTube™ technology was evaluated using 14 Campylobacter jejuni isolated from a commercial turkey flock. The flaA typing was performed using PCR-RFLP with restriction enzymes Sau3AI, AluI, a 'composite' flaA analysis of AluI and Sau3AI and DdeI. The 14 isolates were differentiated into 3, 5, 7 and 9 genotypes, respectively. Entire flaA gene and short variable region (SVR) sequences were analysed. Sequencing of the entire flaA provided 11 different genotypes. flaA-SVR sequence analysis detected 8 flaA alleles and 4 flaA peptides. One new flaA allele type (528) was identified. MLST analysis represented 10 different sequence types (STs) and 5 clonal complexes (CCs). The microarray assay recognised 14 different genotypes. The discriminatory indices were 0.560, 0.802, 0.857, and 0.912 for flaA-RFLP depending on the used enzymes, 0.890 for flaA-SVR, 0.967 for entire flaA sequencing, 0.945 for MLST and 1.00 for the DNA microarray assay. The flaA gene was genetically stable over 20 passages on blood agar. In conclusion, the different typing tools demonstrated a high level of genetic heterogeneity of Campylobacter jejuni in a turkey flock, indicating that a single flock can be infected by multiple genotypes within one rearing cycle. DNA microarray-based assays had the highest discriminatory power when compared with other genotyping tools.
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