ArticleMolecular & cellular proteomics : MCP2013
Parallel visualization of multiple protein complexes in individual cells in tumor tissue.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
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- The Visualization of Protein-Protein Interactions in Breast Cancer: Deployment Study in Pathological Examination.Acta histochemica et cytochemica · 2021Review
- Multiplex protein profiling method for extracellular vesicle protein detection.Scientific reports · 2021Article
- Proximity Ligation Assay as a Tool for Antibody Validation in Human Tissues.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- SYNPLA, a method to identify synapses displaying plasticity after learning.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Visualisation of HER2 homodimers in single cells from HER2 overexpressing primary formalin fixed paraffin embedded tumour tissue.Molecular medicine (Cambridge, Mass.) · 2019Article
- Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay.Journal of visualized experiments : JoVE · 2018Article
- Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes.Scientific reports · 2018Article
- Electrophoretic cytopathology resolves ERBB2 forms with single-cell resolution.NPJ precision oncology · 2018Article
- Additional sex combs interacts with enhancer of zeste and trithorax and modulates levels of trimethylation on histone H3K4 and H3K27 during transcription of hsp70.Epigenetics & chromatin · 2017Article
- Liquid-phase electron microscopy of molecular drug response in breast cancer cells reveals irresponsive cell subpopulations related to lack of HER2 homodimers.Molecular biology of the cell · 2017Article
- Global preamplification simplifies targeted mRNA quantification.Scientific reports · 2017Article
- Detection of Receptor Heteromerization Using In Situ Proximity Ligation Assay.Current protocols in pharmacology · 2016Article
- Let There Be Light!Proteomes · 2016Review
- Detecting individual extracellular vesicles using a multicolor in situ proximity ligation assay with flow cytometric readout.Scientific reports · 2016Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular functions are regulated and executed by complex protein interaction networks. Accordingly, it is essential to understand the interplay between proteins in determining the activity status of signaling cascades. New methods are therefore required to provide information on different protein interaction events at the single cell level in heterogeneous cell populations such as in tissue sections. Here, we describe a multiplex proximity ligation assay for simultaneous visualization of multiple protein complexes in situ. The assay is an enhancement of the original proximity ligation assay, and it is based on using proximity probes labeled with unique tag sequences that can be used to read out which probes, from a pool of probes, have bound a certain protein complex. Using this approach, it is possible to gain information on the constituents of different protein complexes, the subcellular location of the complexes, and how the balance between different complex constituents can change between normal and malignant cells, for example. As a proof of concept, we used the assay to simultaneously visualize multiple protein complexes involving EGFR, HER2, and HER3 homo- and heterodimers on a single-cell level in breast cancer tissue sections. The ability to study several protein complex formations concurrently at single cell resolution could be of great potential for a systems understanding, paving the way for improved disease diagnostics and possibilities for drug development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.