ArticleBiotechnology progress
Very high density of CHO cells in perfusion by ATF or TFF in WAVE bioreactor™. Part I. Effect of the cell density on the process.
Article in Biotechnology progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed.
- High Cell Density Cultivation of HEK293 Cells Using a 2L Membrane-Stirred Bioreactor.Biotechnology and bioengineering · 2026Article
- Perfusion development and its potential for cell therapy manufacturing with adherent cells.Applied microbiology and biotechnology · 2026Review
- Impact of N-1 phase media optimization as a bioprocess intensification strategy on fed-batch production.Bioprocess and biosystems engineering · 2026Article
- Accelerated dynamic characterization of CHO semi-perfusion processes using intensified design of experiments.Bioprocess and biosystems engineering · 2026Article
- Bioreactor Design and Engineering for Cultivated Meat Manufacturing.Advances in biochemical engineering/biotechnology · 2026Review
- Comparative omics profiling reveals differences in biomass, energy production, and vesicle transport between CHO and fast-growing CHL-YN cells.Scientific reports · 2025Article
- MemorySeq identifies heritable epigenetic phenotypes that initiate cell culture stress tolerance in CHO cells.iScience · 2025Article
- Continuous Manufacturing of Recombinant Drugs: Comprehensive Analysis of Cost Reduction Strategies, Regulatory Pathways, and Global Implementation.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Evaluating Apoptotic Gene Efficiency for CHO Culture Performance Using Targeted Integration.ACS synthetic biology · 2025Article
- Computational fluid particle dynamics modeling of tangential flow filtration in perfusion cell culture.Bioprocess and biosystems engineering · 2025Article
- Analysis of cell lysis for improved understanding between the shake tube and stirred tank reactor perfusion CHO cell cultures.Cytotechnology · 2025Article
- Environmental Impacts of Cultured Meat: A Cradle-to-Gate Life Cycle Assessment.ACS food science & technology · 2025Article
- Development of 10 L mass culture system of human induced pluripotent stem cells with intermittent agitation using plastic fluid.Frontiers in bioengineering and biotechnology · 2025Article
- Differentiating erythroblasts adapt to mechanical stimulation by upregulation of cholesterol biosynthesis via S1P/SREBP-induced HMGCR expression.Scientific reports · 2024Article
- Gene Delivery From Granular Scaffolds for Tunable Biologics Manufacturing.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Empirical economic analysis shows cost-effective continuous manufacturing of cultivated chicken using animal-free medium.Nature food · 2024Article
- Adeno-associated virus perfusion enhanced expression: A commercially scalable, high titer, high quality producer cell line process.Molecular therapy. Methods & clinical development · 2024Article
- Effects of process intensification on homogeneity of an IgG1:κ monoclonal antibody during perfusion culture.Applied microbiology and biotechnology · 2024Article
- Cell clone selection-impact of operation modes and medium exchange strategies on clone ranking.Frontiers in bioengineering and biotechnology · 2024Article
- WAVE-based intensified perfusion cell culture for fast process development.Biotechnology letters · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High cell density perfusion process of antibody producing CHO cells was developed in disposable WAVE Bioreactor™ using external hollow fiber filter as cell separation device. Both "classical" tangential flow filtration (TFF) and alternating tangential flow system (ATF) equipment were used and compared. Consistency of both TFF- and ATF-based cultures was shown at 20-35 × 10(6) cells/mL density stabilized by cell bleeds. To minimize the nutrients deprivation and by-product accumulation, a perfusion rate correlated to the cell density was applied. The cells were maintained by cell bleeds at density 0.9-1.3 × 10(8) cells/mL in growing state and at high viability for more than 2 weeks. Finally, with the present settings, maximal cell densities of 2.14 × 10(8) cells/mL, achieved for the first time in a wave-induced bioreactor, and 1.32 × 10(8) cells/mL were reached using TFF and ATF systems, respectively. Using TFF, the cell density was limited by the membrane capacity for the encountered high viscosity and by the pCO2 level. Using ATF, the cell density was limited by the vacuum capacity failing to pull the highly viscous fluid. Thus, the TFF system allowed reaching higher cell densities. The TFF inlet pressure was highly correlated to the viscosity leading to the development of a model of this pressure, which is a useful tool for hollow fiber design of TFF and ATF. At very high cell density, the viscosity introduced physical limitations. This led us to recommend cell densities under 1.46 × 10(8) cell/mL based on the analysis of the theoretical distance between the cells for the present cell line.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.