ArticleJournal of bacteriology2013
Host cell contact induces expression of virulence factors and VieA, a cyclic di-GMP phosphodiesterase, in Vibrio cholerae.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- H-NOX and NosP Regulate Flagellar Protein and Virulence Factor Production in Vibrio Cholerae.Molecular & cellular proteomics : MCP · 2026Article
- Roles of the second messenger c-di-GMP in bacteria: Focusing on the topics of flagellar regulation and Vibrio spp.Genes to cells : devoted to molecular & cellular mechanisms · 2022Review
- On or Off: Life-Changing Decisions Made byInfectious microbes & diseases · 2020Review
- Fur RepressesFrontiers in microbiology · 2020Article
- Vibrio cholerae O1 secretes an extracellular matrix in response to antibody-mediated agglutination.PloS one · 2018Article
- Article
- Engineering microbial physiology with synthetic polymers: cationic polymers induce biofilm formation inChemical science · 2017Article
- BsmR degrades c-di-GMP to modulate biofilm formation of nosocomial pathogen Stenotrophomonas maltophilia.Scientific reports · 2017Article
- The ins and outs of cyclic di-GMP signaling in Vibrio cholerae.Current opinion in microbiology · 2017Review
- Exploiting a host-commensal interaction to promote intestinal barrier function and enteric pathogen tolerance.Science immunology · 2016Article
- Staying Alive: Vibrio cholerae's Cycle of Environmental Survival, Transmission, and Dissemination.Microbiology spectrum · 2016Review
- Cyclo(phenylalanine-proline) induces DNA damage in mammalian cells via reactive oxygen species.Journal of cellular and molecular medicine · 2015Article
- The VieB auxiliary protein negatively regulates the VieSA signal transduction system in Vibrio cholerae.BMC microbiology · 2015Article
- Physiologic Stresses Reveal a Salmonella Persister State and TA Family Toxins Modulate Tolerance to These Stresses.PloS one · 2015Article
- Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vibrio cholerae, a noninvasive bacterium, colonizes the intestinal epithelium and secretes cholera toxin (CT), a potent enterotoxin that causes the severe fluid loss characteristic of the disease cholera. In this study, we demonstrate that adherence of V. cholerae to the intestinal epithelial cell line INT 407 strongly induces the expression of the major virulence genes ctxAB and tcpA and the virulence regulatory gene toxT. No induction of toxR and tcpP, which encode transcriptional activators of toxT, was observed in adhered bacteria, and the adherence-dependent upregulation of toxT expression was independent of ToxR and TcpP. A sharp increase in the expression of the vieA gene, which encodes a cyclic di-GMP (c-di-GMP) phosphodiesterase, was observed in INT 407-adhered V. cholerae immediately after infection. Induction of toxT, ctxAB, and tcpA in INT 407-adhered vieA mutant strain O395 ΔvieA was consistently lower than in the parent strain, although no effect was observed in unadhered bacteria, suggesting that VieA has a role in the upregulation of toxT expression specifically in host cell-adhered V. cholerae. Furthermore, though VieA has both a DNA binding helix-turn-helix domain and an EAL domain conferring c-di-GMP phosphodiesterase activity, the c-di-GMP phosphodiesterase activity of VieA is necessary and sufficient for the upregulation of toxT expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.