ArticleJournal of bacteriology2013
DNA-damaging agents induce the RecA-independent homologous recombination functions of integrating conjugative elements of the SXT/R391 family.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Identification of phage recombinase function unit in genus Corynebacterium.Applied microbiology and biotechnology · 2021Article
- IncC conjugative plasmids and SXT/R391 elements repair double-strand breaks caused by CRISPR-Cas during conjugation.Nucleic acids research · 2020Article
- Replication of the Salmonella Genomic Island 1 (SGI1) triggered by helper IncC conjugative plasmids promotes incompatibility and plasmid loss.PLoS genetics · 2020Article
- Genomic Analysis of SXT/R391 Integrative Conjugative Elements FromFrontiers in microbiology · 2020Article
- Structure and mechanism of the Red recombination system of bacteriophage λ.Progress in biophysics and molecular biology · 2019Review
- Redefinition and Unification of the SXT/R391 Family of Integrative and Conjugative Elements.Applied and environmental microbiology · 2018Article
- The hidden life of integrative and conjugative elements.FEMS microbiology reviews · 2017Review
- Characterization of Three Novel SXT/R391 Integrating Conjugative Elements ICEFrontiers in microbiology · 2016Article
- A λ Cro-Like Repressor Is Essential for the Induction of Conjugative Transfer of SXT/R391 Elements in Response to DNA Damage.Journal of bacteriology · 2015Article
- Transfer activation of SXT/R391 integrative and conjugative elements: unraveling the SetCD regulon.Nucleic acids research · 2015Article
- The extended regulatory networks of SXT/R391 integrative and conjugative elements and IncA/C conjugative plasmids.Frontiers in microbiology · 2015Review
- The master activator of IncA/C conjugative plasmids stimulates genomic islands and multidrug resistance dissemination.PLoS genetics · 2014Article
- Article
- Development of pVCR94ΔX from Vibrio cholerae, a prototype for studying multidrug resistant IncA/C conjugative plasmids.Frontiers in microbiology · 2014Article
- Multiple Pathways of Genome Plasticity Leading to Development of Antibiotic Resistance.Antibiotics (Basel, Switzerland) · 2013Review
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3 authors.
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Abstract
Integrating conjugative elements (ICEs) of the SXT/R391 family are major contributors to the spread of antibiotic resistance genes. These elements also catalyze their own diversity by promoting inter-ICE recombination through the action of the RecA-independent homologous recombination system that they encode. Here, we report that expression of this recombination system, which consists of the single-stranded DNA annealing protein Bet and the exonuclease Exo, is induced by DNA-damaging agents via ICE-encoded transcriptional regulators. We show that the bet and exo genes are part of a large polycistronic transcript that contains many conserved ICE genes that are not involved in the main integration/excision and conjugative transfer processes. We show that although the recombination genes are highly transcribed, their translation is subject to additional strong regulatory mechanisms. We also show that an ICE-encoded putative single-stranded DNA binding protein (Ssb) limits hybrid ICE formation. Finally, a thorough in silico analysis reveals that orthologues of Bet and Exo are widely distributed in bacterial strains belonging to very distantly related bacterial species and are carried by various mobile genetic elements. Phylogenetic analyses suggest that the annealing proteins and exonucleases that compose these systems sometimes have different evolutionary origins, underscoring the strong selective pressure to maintain the functionality of these unrelated cooperating proteins.
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