ArticleJournal of bacteriology2013
Campylobacter jejuni Dps protein binds DNA in the presence of iron or hydrogen peroxide.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Biophysical insights into plasmid DNA binding by deinococcus grandis Dps nanocage.European biophysics journal : EBJ · 2026Article
- Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals.Protein science : a publication of the Protein Society · 2023Article
- Characterization of FA1654: A putative DPS protein in Filifactor alocis.Molecular oral microbiology · 2023Article
- Complementary Roles of Two DNA Protection Proteins fromInternational journal of molecular sciences · 2022Article
- Dps Is a Universally Conserved Dual-Action DNA-Binding and Ferritin Protein.Journal of bacteriology · 2022Review
- The Conformation of the N-Terminal Tails ofInternational journal of molecular sciences · 2022Article
- Role of the Filifactor alocis Hypothetical Protein FA519 in Oxidative Stress Resistance.Microbiology spectrum · 2021Article
- Dps-DNA interaction in Marinobacter hydrocarbonoclasticus protein: effect of a single-charge alteration.European biophysics journal : EBJ · 2021Article
- Dps Protects Enterohemorrhagic Escherichia coli against Acid-Induced Antimicrobial Peptide Killing.Journal of bacteriology · 2020Article
- Article
- The Oligomeric Form of the Escherichia coli Dps Protein Depends on the Availability of Iron Ions.Molecules (Basel, Switzerland) · 2017Article
- Oxidative and nitrosative stress defences of Helicobacter and Campylobacter species that counteract mammalian immunity.FEMS microbiology reviews · 2016Article
- Study on the mechanism of antibacterial action of magnesium oxide nanoparticles against foodborne pathogens.Journal of nanobiotechnology · 2016Article
- Identification of putative adhesins of Actinobacillus suis and their homologues in other members of the family Pasteurellaceae.BMC research notes · 2015Article
- The DNA-Binding Protein from Starved Cells (Dps) Utilizes Dual Functions To Defend Cells against Multiple Stresses.Journal of bacteriology · 2015Article
- A ferritin-like protein with antioxidant activity in Ureaplasma urealyticum.BMC microbiology · 2015Article
- Regulation of oxidative stress resistance in Campylobacter jejuni, a microaerophilic foodborne pathogen.Frontiers in microbiology · 2015Review
- Modes of Escherichia coli Dps Interaction with DNA as Revealed by Atomic Force Microscopy.PloS one · 2015Article
- A tale of tails: deciphering the contribution of terminal tails to the biochemical properties of two Dps proteins from Streptomyces coelicolor.Cellular and molecular life sciences : CMLS · 2014Article
- The three-dimensional structure of the extracellular adhesion domain of the sialic acid-binding adhesin SabA from Helicobacter pylori.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Iron is an essential cofactor for many enzymes; however, this metal can lead to the formation of reactive oxygen species. Ferritin proteins bind and oxidize Fe(2+) to Fe(3+), storing this metal in a nonreactive form. In some organisms, a particular subfamily of ferritins, namely, Dps proteins, have the ability to bind DNA. Here we show that the Campylobacter jejuni Dps has DNA binding activity that is uniquely activated by Fe(2+) or H2O2 at below neutral pH. The Dps-DNA binding activity correlated with the ability of Dps to self-aggregate. The Dps-DNA interaction was inhibited by NaCl and Mg(2+), suggesting the formation of ionic interactions between Dps and DNA. Alkylation of cysteines affected DNA binding in the presence of H2O2 but not in the presence of Fe(2+). Replacement of all cysteines in C. jejuni Dps with serines did not affect DNA binding, excluding the participation of cysteine in H2O2 sensing. Dps was able to protect DNA in vitro from enzymatic cleavage and damage by hydroxyl radicals. A C. jejuni dps mutant was less resistant to H2O2 in vivo. The concerted activation of Dps-DNA binding in response to low pH, H2O2, and Fe(2+) may protect C. jejuni DNA during host colonization.
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