ArticleApplied and environmental microbiology2013
Comparative transcriptomic analysis of the Burkholderia cepacia tyrosine kinase bceF mutant reveals a role in tolerance to stress, biofilm formation, and virulence.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Rearing Management and Its Impact on Caseous Lymphadenitis in Sheep.Animals : an open access journal from MDPI · 2024Article
- Biofilm Signaling, Composition and Regulation inJournal of microbiology and biotechnology · 2023Review
- Structural and Functional Insights into the Biofilm-Associated BceF Tyrosine Kinase Domain fromBiomolecules · 2021Article
- Drought-induced soil microbial amino acid and polysaccharide change and their implications for C-N cycles in a climate change world.Scientific reports · 2019Article
- The OmpR Regulator of Burkholderia multivorans Controls Mucoid-to-Nonmucoid Transition and Other Cell Envelope Properties Associated with Persistence in the Cystic Fibrosis Lung.Journal of bacteriology · 2018Article
- c-di-GMP Regulates Various Phenotypes and Insecticidal Activity of Gram-PositiveFrontiers in microbiology · 2018Article
- Key Players and Individualists of Cyclic-di-GMP Signaling inFrontiers in microbiology · 2018Article
- Nitrate Sensing and Metabolism Inhibit Biofilm Formation in the Opportunistic PathogenFrontiers in microbiology · 2017Article
- Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing.Virulence · 2016Review
- Tyrosine Phosphorylation and Dephosphorylation in Burkholderia cenocepacia Affect Biofilm Formation, Growth under Nutritional Deprivation, and Pathogenicity.Applied and environmental microbiology · 2016Article
- Electrochemical impedance immunosensor for rapid detection of stressed pathogenic Staphylococcus aureus bacteria.Environmental science and pollution research international · 2015Article
- Protein kinase A (PknA) of Mycobacterium tuberculosis is independently activated and is critical for growth in vitro and survival of the pathogen in the host.The Journal of biological chemistry · 2015Article
- The tyrosine kinase BceF and the phosphotyrosine phosphatase BceD of Burkholderia contaminans are required for efficient invasion and epithelial disruption of a cystic fibrosis lung epithelial cell line.Infection and immunity · 2015Article
- Microbial protein-tyrosine kinases.The Journal of biological chemistry · 2014Review
- Nitropropenyl benzodioxole, an anti-infective agent with action as a protein tyrosine phosphatase inhibitor.The open medicinal chemistry journal · 2014Article
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
Abstract
The bacterial tyrosine-kinase (BY-kinase) family comprises the major group of bacterial enzymes endowed with tyrosine kinase activity. We previously showed that the BceF protein from Burkholderia cepacia IST408 belongs to this BY-kinase family and is involved in the biosynthesis of the exopolysaccharide cepacian. However, little is known about the extent of regulation of this protein kinase activity. In order to examine this regulation, we performed a comparative transcriptome profile between the bceF mutant and wild-type B. cepacia IST408. The analyses led to identification of 630 genes whose expression was significantly changed. Genes with decreased expression in the bceF mutant were related to stress response, motility, cell adhesion, and carbon and energy metabolism. Genes with increased expression were related to intracellular signaling and lipid metabolism. Mutation of bceF led to reduced survival under heat shock and UV light exposure, reduced swimming motility, and alteration in biofilm architecture when grown in vitro. Consistent with some of these phenotypes, the bceF mutant demonstrated elevated levels of cyclic-di-GMP. Furthermore, BceF contributed to the virulence of B. cepacia for larvae of the Greater wax moth, Galleria mellonella. Taken together, BceF appears to play a considerable role in many cellular processes, including biofilm formation and virulence. As homologues of BceF occur in a number of pathogenic and plant-associated Burkholderia strains, the modulation of bacterial behavior through tyrosine kinase activity is most likely a widely occurring phenomenon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.