ArticleApplied and environmental microbiology2013
Simultaneous quantification of multiple food- and waterborne pathogens by use of microfluidic quantitative PCR.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed.
- Trial
- Yes/No Quantitative Analysis of Single-Stranded Oligonucleotides with Lateral Flow Assays.Biosensors · 2026Article
- Ecological Health and Freshwater Pathogen Using eDNA Metabarcoding: A Preliminary Assessment for Environmental Surveillance Development in Malaysia.Microorganisms · 2025Article
- Validation of a High-Throughput Microfluidic Real-Time PCR for the Detection of Vector-Borne Agents in Wild Birds from the Brazilian Pantanal.Pathogens (Basel, Switzerland) · 2025Article
- Stochastic generalization models learn to comprehensively detect volatile organic compounds associated with foodborne pathogensRSC advances · 2025Article
- Recent developments in waterborne pathogen detection technologies.Environmental monitoring and assessment · 2025Review
- Microfluidics for detection of food pathogens: recent trends and opportunities.Journal of food science and technology · 2024Review
- Impacts of biostimulation and bioaugmentation on woodchip bioreactor microbiomes.Microbiology spectrum · 2023Article
- High-Throughput Microfluidic Quantitative PCR Platform for the Simultaneous Quantification of Pathogens, Fecal Indicator Bacteria, and Microbial Source Tracking Markers.ACS ES&T water · 2023Article
- Assessment of pathogens in flood waters in coastal rural regions: Case study after Hurricane Michael and Florence.PloS one · 2023Article
- Temporal assessment of N-cycle microbial functions in a tropical agricultural soil using gene co-occurrence networks.PloS one · 2023Article
- Application of a high-throughput quantitative PCR system for simultaneous monitoring of SARS-CoV-2 variants and other pathogenic viruses in wastewater.The Science of the total environment · 2022Article
- The Efficient and Practical virus Identification System with ENhanced Sensitivity for Solids (EPISENS-S): A rapid and cost-effective SARS-CoV-2 RNA detection method for routine wastewater surveillance.The Science of the total environment · 2022Article
- Antimicrobial Resistance Monitoring of Water Environments: A Framework for Standardized Methods and Quality Control.Environmental science & technology · 2022Review
- The Microbial Nitrogen Cycling, Bacterial Community Composition, and Functional Potential in a Natural Grassland Are Stable from Breaking Dormancy to Being Dormant Again.Microorganisms · 2022Article
- High-throughput qPCR and 16S rRNA gene amplicon sequencing as complementary methods for the investigation of the cheese microbiota.BMC microbiology · 2022Article
- Stoichiometric approach to quantitative analysis of biomolecules: the case of nucleic acids.Analytical and bioanalytical chemistry · 2022Article
- Universal Enzyme-Based Field Workflow for Rapid and Sensitive Quantification of Water Pathogens.Microorganisms · 2021Article
- The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis.Iranian journal of public health · 2021Review
- Gut microbiota response to antibiotics is personalized and depends on baseline microbiota.Microbiome · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The direct quantification of multiple pathogens has been desired for diagnostic and public health purposes for a long time. In this study, we applied microfluidic quantitative PCR (qPCR) technology to the simultaneous detection and quantification of multiple food- and waterborne pathogens. In this system, multiple singleplex qPCR assays were run under identical detection conditions in nanoliter-volume chambers that are present in high densities on a chip. First, we developed 18 TaqMan qPCR assays that could be run in the same PCR conditions by using prevalidated TaqMan probes. Specific and sensitive quantification was achieved by using these qPCR assays. With the addition of two previously validated TaqMan qPCR assays, we used 20 qPCR assays targeting 10 enteric pathogens, a fecal indicator bacterium (general Escherichia coli), and a process control strain in the microfluidic qPCR system. We preamplified the template DNA to increase the sensitivity of the qPCR assays. Our results suggested that preamplification was effective for quantifying small amounts of the template DNA without any major impact on the sensitivity, efficiency, and quantitative performance of qPCR. This microfluidic qPCR system allowed us to detect and quantify multiple pathogens from fecal samples and environmental water samples spiked with pathogens at levels as low as 100 cells/liter. These results suggest that the routine monitoring of multiple pathogens in food and water samples is now technically feasible. This method may provide more reliable information for risk assessment than the current fecal contamination indicator approach.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.