ArticleTransgenic research2013
An optimized sericin-1 expression system for mass-producing recombinant proteins in the middle silk glands of transgenic silkworms.
Article in Transgenic research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 67 citations in OpenAlex.
- Novel GS5 sericin mitigates UVA-induced photoaging by activating Nrf2 and inhibiting the JAK-STAT pathway.International journal of biological sciences · 2026Article
- Biosynthesis of a dual growth factors (GFs) functionalized silk sericin hydrogel to promote chronic wound healing in diabetic mice.Bioactive materials · 2025Article
- Biosynthesis of Bioactive Human Neurotrophic Factor 3 in Silkworms and Its Biomedical Applications.Insects · 2025Article
- High mechanical property silk produced by transgenic silkworms expressing theFrontiers in bioengineering and biotechnology · 2024Article
- A novel method for silkworm cocoons self-degumming and its effect on silk fibers.Journal of advanced research · 2023Article
- A Bioengineering Approach for the Development of Fibroblast Growth Factor-7-Functionalized Sericin Biomaterial Applicable for the Cultivation of Keratinocytes.International journal of molecular sciences · 2022Article
- Bioengineered Silkworm for Producing Cocoons with High Fibroin Content for Regenerated Fibroin Biomaterial-Based Applications.International journal of molecular sciences · 2022Article
- Bibliometric Analysis of Trends in Mulberry and Silkworm Research on the Production of Silk and Its By-Products.Insects · 2022Review
- Bioengineered silkworm model for expressing human neurotrophin-4 with potential biomedical application.Frontiers in physiology · 2022Article
- Effects of transgenic silk materials that incorporate FGF-7 protein microcrystals on the proliferation and differentiation of human keratinocytes.FASEB bioAdvances · 2020Article
- Transdermal peptide conjugated to human connective tissue growth factor with enhanced cell proliferation and hyaluronic acid synthesis activities produced by a silkworm silk gland bioreactor.Applied microbiology and biotechnology · 2020Article
- The beta-1, 4-N-acetylglucosaminidase 1 gene, selected by domestication and breeding, is involved in cocoon construction of Bombyx mori.PLoS genetics · 2020Article
- Effects of Osiris9a on Silk Properties inInternational journal of molecular sciences · 2020Article
- CRISPR/Cas12a Mediated Genome Editing EnhancesFrontiers in bioengineering and biotechnology · 2020Article
- Expression and characterization of recombinant human VEGF165 in the middle silk gland of transgenic silkworms.Transgenic research · 2019Article
- Insights into the regulatory characteristics of silkworm fibroin gene promoters using a modified Gal4/UAS system.Transgenic research · 2019Article
- Optimization of a 2A self-cleaving peptide-based multigene expression system for efficient expression of upstream and downstream genes in silkworm.Molecular genetics and genomics : MGG · 2019Article
- A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities.Journal of biological engineering · 2019Article
- Transgenic Silkworm-Based Silk Gland Bioreactor for Large Scale Production of Bioactive Human Platelet-Derived Growth Factor (PDGF-BB) in Silk Cocoons.International journal of molecular sciences · 2018Article
- High-efficiency production of human serum albumin in the posterior silk glands of transgenic silkworms, Bombyx mori L.PloS one · 2018Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The middle silk gland (MSG) of silkworm is thought to be a potential host for mass-producing valuable recombinant proteins. Transgenic MSG expression systems based on the usage of promoter of sericin1 gene (sericin-1 expression system) have been established to produce various recombinant proteins in MSG. However, further modifying the activity of the sericin-1 expression system to yield higher amounts of recombinant proteins is still necessary. In this study, we provide an alternative modification strategy to construct an efficient sericin-1 expression system by using the hr3 enhancer (hr3 CQ) from a Chongqing strain of the Bombyx mori nuclear polyhedrosis virus (BmNPV) and the 3'UTRs of the fibroin heavy chain (Fib-HPA), the fibroin light chain (Fib-LPA), and Sericin1 (Ser1PA) genes. We first analyzed the effects of these DNA elements on expression of luciferase, and found that the combination of hr3 CQ and Ser1PA was most effective to increase the activity of luciferase. Then, hr3 CQ and Ser1PA were used to modify the sericin1 expression system. Transgenic silkworms bearing these modified sericin1 expression vectors were generated by a piggyBac transposon mediated genetic transformation method. Our results showed that mRNA level of DsRed reporter gene in transgenic silkworms containing hr3 CQ and Ser1PA significantly increased by 9 fold to approximately 83 % of that of endogenous sericin1. As the results of that, the production of recombinant RFP increased by 16 fold to 9.5 % (w/w) of cocoon shell weight. We conclude that this modified sericin-1 expression system is efficient and will contribute to the MSG as host to mass produce valuable recombinant proteins.
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