ArticleOncogene2014
Selective regulation of p38β protein and signaling by integrin-linked kinase mediates bladder cancer cell migration.
Article in Oncogene, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 0 citations in OpenAlex.
- Prognosis and predictive value of heat-shock proteins expression in oral cancer: A PRISMA-compliant meta-analysis.Medicine · 2021Pooled it
- Comprehensive analysis of heat shock proteins in glioma revealed the association with glioma-associated myeloid cells.Genes and immunity · 2025Article
- HSF1 and Its Role in Huntington's Disease Pathology.Advances in experimental medicine and biology · 2023Review
- The kinase activity of integrin-linked kinase regulates cellular senescence in gastric cancer.Cell death & disease · 2022Article
- Atractylenolide I inhibits EMT and enhances the antitumor effect of cabozantinib in prostate cancerFrontiers in oncology · 2022Article
- p38β - MAPK11 and its role in female cancers.Journal of ovarian research · 2021Review
- p38β and Cancer: The Beginning of the Road.International journal of molecular sciences · 2020Review
- Integrin-Linked Kinase Is Involved In the Proliferation and Invasion of Esophageal Squamous Cell Carcinoma.Journal of Cancer · 2020Article
- Rsu1-dependent control of PTEN expression is regulated via ATF2 and cJun.Journal of cell communication and signaling · 2019Article
- MICAL-L2 potentiates Cdc42-dependent EGFR stability and promotes gastric cancer cell migration.Journal of cellular and molecular medicine · 2019Article
- A functional CRISPR/Cas9 screen identifies kinases that modulate FGFR inhibitor response in gastric cancer.Oncogenesis · 2019Article
- Targeting Heat Shock Proteins in Cancer: A Promising Therapeutic Approach.International journal of molecular sciences · 2017Review
- Gelsolin suppresses gastric cancer metastasis through inhibition of PKR-p38 signaling.Oncotarget · 2016Article
- Molecular networks underlying myofibroblast fate and fibrosis.Journal of molecular and cellular cardiology · 2016Review
- Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- p38 MAP kinases in the heart.Gene · 2016Review
- Transcriptional Activation of Inflammatory Genes: Mechanistic Insight into Selectivity and Diversity.Biomolecules · 2015Review
- Integrin β3 Haploinsufficiency Modulates Serotonin Transport and Antidepressant-Sensitive Behavior in Mice.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2015Article
- Protein interactome of muscle invasive bladder cancer.PloS one · 2015Article
- Mammalian target of rapamycin complex 2 (mTORC2) is a critical determinant of bladder cancer invasion.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Integrin-linked kinase (ILK) and p38(MAPK) are protein kinases that transduce extracellular signals regulating cell migration and actin cytoskeletal organization. ILK-dependent regulation of p38(MAPK) is critical for mammalian kidney development and in smooth muscle cell migration, however, specific p38 isoforms has not been previously examined in ILK-regulated responses. Signaling by ILK and p38(MAPK) is often dysregulated in bladder cancer, and here we report a strong positive correlation between protein levels of ILK and p38β, which is the predominant isoform found in bladder cancer cells, as well as in patient-matched normal bladder and tumor samples. Knockdown by RNA interference of either p38β or ILK disrupts serum-induced, Rac1-dependent migration and actin cytoskeletal organization in bladder cancer cells. Surprisingly, ILK knockdown causes the selective reduction in p38β cellular protein level, without inhibiting p38β messenger RNA (mRNA) expression. The loss of p38β protein in ILK-depleted cells is partially rescued by the 26S proteasomal inhibitor MG132. Using co-precipitation and bimolecular fluorescent complementation assays, we find that ILK selectively forms cytoplasmic complexes with p38β. In situ proximity ligation assays further demonstrate that serum-stimulated assembly of endogenous ILK-p38β complexes is sensitive to QLT-0267, a small molecule ILK kinase inhibitor. Finally, inhibition of ILK reduces the amplitude and period of serum-induced activation of heat shock protein 27 (Hsp27), a target of p38β implicated in actin cytoskeletal reorganization. Our work identifies Hsp27 as a novel target of ILK-p38β signaling complexes, playing a key role in bladder cancer cell migration.
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