ArticleMedical science monitor basic research2013
Effects of verapamil on the immediate-early gene expression of bone marrow mesenchymal stem cells stimulated by mechanical strain in vitro.
Article in Medical science monitor basic research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Tensile strain promotes osteogenic differentiation of bone marrow mesenchymal stem cells through upregulating lncRNA-MEG3.Histology and histopathology · 2021Article
- Review
- Comparison of hIGF-1 gene transfection to the hBMSCs and human meniscal fibrochondrocytes.Medical science monitor : international medical journal of experimental and clinical research · 2015Article
- Dual AO/EB staining to detect apoptosis in osteosarcoma cells compared with flow cytometry.Medical science monitor basic research · 2015Article
- Mechanical strain regulates osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells.BioMed research international · 2015Article
- Prostacyclin suppresses twist expression in the presence of indomethacin in bone marrow-derived mesenchymal stromal cells.Medical science monitor : international medical journal of experimental and clinical research · 2014Article
- Mechanical stress stimulates the osteo/odontoblastic differentiation of human stem cells from apical papilla via erk 1/2 and JNK MAPK pathways.BioMed research international · 2014Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo study the effects of verapamil on the immediate-early genes (IEGs) expression of bone marrow mesenchymal stem cells (MSCs) stimulated by cyclic mechanical strain, in order to deduce the role of calcium ion channel in the cell signaling responses of MSCs to mechanical strain. MATERIAL AND
methodsMSCs were isolated and cultured, and the passage of 3-6 MSCs were stimulated by mechanical strain and pretreated with or without verapamil. After that, flow cytometry was used to measure the fluorescence intensity of intracellular Ca(2+) immediately. The expression of early-response genes/proteins (c-fos, c-jun and c-myc) were examined by RT-PCR, immunohistochemistry and Western blot.
resultsIntracellular Ca(2+) concentration of MSCs significantly changed when stimulated by cyclic strain, and the expression of c-fos, c-jun and c-myc remarkably increased in both mRNA and protein levels, while verapamil pre-treatment partially inhibited these effects (P<0.01).
conclusionsThe changes of the intracellular calcium concentration of MSCs induced by mechanical strain, dependent on the regulation of calcium channel activation, might play a role in the early response of MSCs to cyclic strain.
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