ArticleNucleic acids research2013
The RNA-binding region of human TRBP interacts with microRNA precursors through two independent domains.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- Synthetic nanoparticles for cell-type specific, spatially resolved loading and export of MiRNAs in neural cells.Journal of nanobiotechnology · 2026Article
- Article
- Extracellular Vesicle MicroRNAs in Heart Failure: Pathophysiological Mediators and Therapeutic Targets.Cells · 2023Review
- Inherent conformational plasticity in dsRBDs enables interaction with topologically distinct RNAs.Biophysical journal · 2022Article
- The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity.Nucleic acids research · 2022Article
- Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless.Nucleic acids research · 2019Article
- Structural basis of siRNA recognition by TRBP double-stranded RNA binding domains.The EMBO journal · 2018Article
- Conserved asymmetry underpins homodimerization of Dicer-associated double-stranded RNA-binding proteins.Nucleic acids research · 2017Article
- DRB4 dsRBD1 drives dsRNA recognition in Arabidopsis thaliana tasi/siRNA pathway.Nucleic acids research · 2017Article
- RNA Scanning of a Molecular Machine with a Built-in Ruler.Journal of the American Chemical Society · 2017Article
- TRBP ensures efficient Dicer processing of precursor microRNA in RNA-crowded environments.Nature communications · 2016Article
- S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.Nucleic acids research · 2016Article
- SUMOylation of TARBP2 regulates miRNA/siRNA efficiency.Nature communications · 2015Article
- Dicer-TRBP complex formation ensures accurate mammalian microRNA biogenesis.Molecular cell · 2015Article
- Article
- Down-regulation of the antisense mitochondrial non-coding RNAs (ncRNAs) is a unique vulnerability of cancer cells and a potential target for cancer therapy.The Journal of biological chemistry · 2014Article
- Derivation and characterization of Dicer- and microRNA-deficient human cells.RNA (New York, N.Y.) · 2014Article
- Dissecting the roles of TRBP and PACT in double-stranded RNA recognition and processing of noncoding RNAs.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression through RNA interference. Human miRNAs are generated through a series of enzymatic processing steps. The precursor miRNA (pre-miRNA) is recognized and cleaved by a complex containing Dicer and several non-catalytic accessory proteins. HIV TAR element binding protein (TRBP) is a constituent of the Dicer complex, which augments complex stability and potentially functions in substrate recognition and product transfer to the RNA-induced silencing complex. Here we have analysed the interaction between the RNA-binding region of TRBP and an oncogenic human miRNA, miR-155, at different stages in the biogenesis pathway. We show that the region of TRBP that binds immature miRNAs comprises two independent double-stranded RNA-binding domains connected by a 60-residue flexible linker. No evidence of contact between the two double-stranded RNA-binding domains was observed either in the apo- or RNA-bound state. We establish that the RNA-binding region of TRBP interacts with both pre-miR-155 and the miR-155/miR-155* duplex through the same binding surfaces and with similar affinities, and that two protein molecules can simultaneously interact with each immature miRNA. These data suggest that TRBP could play a role before and after processing of pre-miRNAs by Dicer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.