ArticleInternational journal of molecular sciences2013
Expression of a functional recombinant human basic fibroblast growth factor from transgenic rice seeds.
Article in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 44 citations in OpenAlex.
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- Biopotentials of Collagen Scaffold Impregnated with Plant-Cell-Derived Epidermal Growth Factor in Defective Bone Healing.Materials (Basel, Switzerland) · 2023Article
- High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor inPlant biotechnology (Tokyo, Japan) · 2022Article
- Production of Recombinant Active Human TGFβ1 inFrontiers in plant science · 2022Article
- Molecular Pharming of the Recombinant Protein hEGF-hEGF Concatenated with Oleosin Using TransgenicGenes · 2020Article
- Plant Molecular Farming: A Viable Platform for Recombinant Biopharmaceutical Production.Plants (Basel, Switzerland) · 2020Review
- Local supplementation with plant-derived recombinant human FGF2 protein enhances bone formation in critical-sized calvarial defects.Journal of bone and mineral metabolism · 2019Article
- Article
- Rice endosperm is cost-effective for the production of recombinant griffithsin with potent activity against HIV.Plant biotechnology journal · 2016Article
- Rice endosperm produces an underglycosylated and potent form of the HIV-neutralizing monoclonal antibody 2G12.Plant biotechnology journal · 2016Article
- Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco.International journal of molecular sciences · 2015Article
- Transgenic rice endosperm as a bioreactor for molecular pharming.Plant cell reports · 2014Review
- Immunotoxicity assessment of rice-derived recombinant human serum albumin using human peripheral blood mononuclear cells.PloS one · 2014Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Basic fibroblast growth factor (FGF-2) is an important member of the FGF gene family. It is widely used in clinical applications for scald and wound healing in order to stimulate cell proliferation. Further it is applied for inhibiting stem cell differentiation in cultures. Due to a shortage of plasma and low expression levels of recombinant rbFGF in conventional gene expression systems, we explored the production of recombinant rbFGF in rice grains (Oryza sativa bFGF, OsrbFGF). An expression level of up to 185.66 mg/kg in brown rice was obtained. A simple purification protocol was established with final recovery of 4.49% and resulting in a yield of OsrbFGF reaching up to 8.33 mg/kg OsrbFGF. The functional assay of OsrbFGF indicated that the stimulating cell proliferation activity on NIH/3T3 was the same as with commercialized rbFGF. Wound healing in vivo of OsrbFGF is equivalent to commercialized rbFGF. Our results indicate that rice endosperm is capable of expressing small molecular mass proteins, such as bFGF. This again demonstrates that rice endosperm is a promising system to express various biopharmaceutical proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.