ArticlePloS one2013
Resistance to the novel fungicide pyrimorph in Phytophthora capsici: risk assessment and detection of point mutations in CesA3 that confer resistance.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 93 citations in OpenAlex.
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- Antifungal spectrum of cyclobutrifluram and multi-point mutations in CcSdh proteins confer resistance in Corynespora cassiicola.Stress biology · 2025Article
- Deciphering fungicide resistance in Phytophthora: mechanisms, prevalence, and sustainable management approaches.World journal of microbiology & biotechnology · 2024Review
- Characterization of PcSTT3B as a Key Oligosaccharyltransferase Subunit Involved in N-glycosylation and Its Role in Development and Pathogenicity ofInternational journal of molecular sciences · 2023Article
- Discovery of Novel Cinnamide Fungicidal Leads with Optical Hydroxyl Side Chain.Molecules (Basel, Switzerland) · 2022Article
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- Preliminary Study of Resistance Mechanism ofMolecules (Basel, Switzerland) · 2022Article
- Functional Analysis of the C-5 Sterol Desaturase PcErg3 in the Sterol Auxotrophic Oomycete PathogenFrontiers in microbiology · 2022Article
- Stepwise accumulation of mutations in CesA3 inEvolutionary applications · 2021Article
- Catalytic Reactions and Energy Conservation in the CytochromeChemical reviews · 2021Review
- Design, synthesis and antifungal activity of threoninamide carbamate derivatives via pharmacophore model.Journal of enzyme inhibition and medicinal chemistry · 2020Article
- Transcriptome analysis clarified genes involved in resistance to Phytophthora capsici in melon.PloS one · 2020Article
- Sensitivity of Different Developmental Stages and Resistance Risk Assessment ofFrontiers in microbiology · 2020Article
- Cellulose Nanocrystal Surface Cationization: A New Fungicide with High Activity againstMolecules (Basel, Switzerland) · 2019Article
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- Evidence of genetically diverse virulent mating types of Phytophthora capsici from Capsicum annum L.World journal of microbiology & biotechnology · 2018Article
- Quantitative proteomics links metabolic pathways to specific developmental stages of the plant-pathogenic oomycete Phytophthora capsici.Molecular plant pathology · 2017Article
- Assessing the Risk for Resistance and Elucidating the Genetics ofFrontiers in microbiology · 2017Article
- A complex game of hide and seek: the search for new antifungals.MedChemComm · 2016Article
- Resistance Assessment for Oxathiapiprolin in Phytophthora capsici and the Detection of a Point Mutation (G769W) in PcORP1 that Confers Resistance.Frontiers in microbiology · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyrimorph is a novel fungicide with high activity against the plant pathogen Phytophthora capsici. We investigated the risk that P. capsici can develop resistance to pyrimorph. The baseline sensitivities of 226 P. capsici isolates, tested by mycelial growth inhibition, showed a unimodal distribution with a mean EC(50) value of 1.4261 (± 0.4002) µg/ml. Twelve pyrimorph-resistant mutants were obtained by repeated exposure to pyrimorph in vitro with a frequency of approximately 1 × 10(-4). The resistance factors of the mutants ranged from 10.67 to 56.02. Pyrimorph resistance of the mutants was stable after 10 transfers on pyrimorph-free medium. Fitness in sporulation, cystospore germination, and pathogenicity in the pyrimorph-resistant mutants was similar to or less than that in the parental wild-type isolates. On detached pepper leaves and pepper plants treated with the recommended maximum dose of pyrimorph, however, virulence was greater for mutants with a high level of pyrimorph resistance than for the wild type. The results suggest that the risk of P. capsici developing resistance to pyrimorph is low to moderate. Among mutants with a high level of pyrimorph resistance, EC(50) values for pyrimorph and CAA fungicides flumorph, dimethomorph, and mandipropamid were positively correlated. This indicated that point mutations in cellulose synthase 3 (CesA3) may confer resistance to pyrimorph. Comparison of CesA3 in isolates with a high level of pyrimorph resistance and parental isolates showed that an amino acid change from glutamine to lysine at position 1077 resulted in stable, high resistance in the mutants. Based on the point mutations, an allele-specific PCR method was developed to detect pyrimorph resistance in P. capsici populations.
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