ArticleProceedings of the National Academy of Sciences of the United States of America2013
Zebrafish model for allogeneic hematopoietic cell transplantation not requiring preconditioning.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Zebrafish: A Relevant Genetic Model for Human Primary Immunodeficiency (PID) Disorders?International journal of molecular sciences · 2023Review
- Generation and Characterization of a Zebrafish IL-2Rγc SCID Model.International journal of molecular sciences · 2022Article
- Article
- Excessive R-loops trigger an inflammatory cascade leading to increased HSPC production.Developmental cell · 2021Article
- A novel conditioning-free hematopoietic stem cell transplantation model in zebrafish.Blood advances · 2020Article
- Article
- Hematopoiesis: A Layered Organization Across Chordate Species.Frontiers in cell and developmental biology · 2020Review
- Enrichment of hematopoietic stem/progenitor cells in the zebrafish kidney.Scientific reports · 2019Article
- Article
- Inducible disruption of the c-myb gene allows allogeneic bone marrow transplantation without irradiation.Journal of immunological methods · 2018Article
- Clonal fate mapping quantifies the number of haematopoietic stem cells that arise during development.Nature cell biology · 2017Article
- T Cell Immune Deficiency inMolecular and cellular biology · 2016Article
- Understanding the regulation of vertebrate hematopoiesis and blood disorders - big lessons from a small fish.FEBS letters · 2016Review
- Stable multilineage xenogeneic replacement of definitive hematopoiesis in adult zebrafish.Scientific reports · 2016Article
- Repairing quite swimmingly: advances in regenerative medicine using zebrafish.Disease models & mechanisms · 2014Review
- zebraflash transgenic lines for in vivo bioluminescence imaging of stem cells and regeneration in adult zebrafish.Development (Cambridge, England) · 2013Article
- Assaying hematopoiesis using zebrafish.Blood cells, molecules & diseases · 2013Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent work on vertebrate hematopoiesis has uncovered the presence of deeply rooted similarities between fish and mammals at molecular and cellular levels. Although small animal models such as zebrafish are ideally suited for genetic and chemical screens, the study of cellular aspects of hematopoietic development in lower vertebrates is severely hampered by the complex nature of their histocompatibility-determining genes. Hence, even when hosts are sublethally irradiated before hematopoietic cell transplantation, stable and long-term reconstitution by allogeneic stem cells often fails. Here, we describe the unexpected observation that transplantation and maintenance of allogeneic hematopoietic stem cells in zebrafish homozygous for the c-myb(t25127) allele, carrying a missense mutation (Ile181Asn) in the DNA binding domain can be achieved without prior conditioning. Using this model, we examined several critical parameters of zebrafish hematopoiesis in a near-physiological setting. Limiting dilution analysis suggests that the kidney marrow of adult zebrafish harbors about 10 transplantable hematopoietic stem cells; this tissue also contains thymus-settling precursors that colonize the thymic rudiment within days after transplantation and initiate robust T-cell development. We also demonstrate that c-myb mutants can be stably reconstituted with hematopoietic cells carrying specific genetic defects in lymphocyte development, exemplifying one of the many potential uses of this model in experimental hematology.
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