ArticleProceedings of the National Academy of Sciences of the United States of America2013
Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 178 citations in OpenAlex.
- Hybrid metabolic engineering enables xylose-driven co-production of polyhydroxybutyrate and violacein in Escherichia coli.Journal of biological engineering · 2026Article
- Base editor-mediated large-scale screening of functional mutations in bacteria for industrial phenotypes.Science China. Life sciences · 2024Article
- Enhanced bioethanol production by evolved Escherichia coli LGE2-H in a microbial electrolysis cell system.Bioresources and bioprocessing · 2024Article
- Mechanism of furfural toxicity and metabolic strategies to engineer tolerance in microbial strains.Microbial cell factories · 2023Review
- Whole-Genome Sequence and Fermentation Characteristics ofBioengineering (Basel, Switzerland) · 2023Article
- Enhanced tolerance of Cupriavidus necator NCIMB 11599 to lignocellulosic derived inhibitors by inserting NAD salvage pathway genes.Bioprocess and biosystems engineering · 2022Article
- Extrapolation of design strategies for lignocellulosic biomass conversion to the challenge of plastic waste.Journal of industrial microbiology & biotechnology · 2022Article
- Fuelling the future of sustainable sugar fermentation across generations.Engineering biology · 2022Review
- Microbial detoxification of lignocellulosic biomass hydrolysates: Biochemical and molecular aspects, challenges, exploits and future perspectives.Frontiers in bioengineering and biotechnology · 2022Review
- Unraveling the mechanism of furfural tolerance in engineeredFrontiers in microbiology · 2022Article
- Effects ofFrontiers in bioengineering and biotechnology · 2022Article
- Inhibitor tolerance and bioethanol fermentability of levoglucosan-utilizingSynthetic and systems biotechnology · 2021Article
- Efficient lactic acid production from dilute acid-pretreated lignocellulosic biomass by a synthetic consortium of engineered Pseudomonas putida and Bacillus coagulans.Biotechnology for biofuels · 2021Article
- Overexpression of Oxidoreductase YghA Confers Tolerance of Furfural in Ethanologenic Escherichia coli Strain SSK42.Applied and environmental microbiology · 2021Article
- A review on enzymes and pathways for manufacturing polyhydroxybutyrate from lignocellulosic materials.3 Biotech · 2021Review
- Mutations in adaptively evolved Escherichia coli LGE2 facilitated the cost-effective upgrading of undetoxified bio-oil to bioethanol fuel.Bioresources and bioprocessing · 2021Article
- Metabolic engineering of Escherichia coli for the production of isobutanol: a review.World journal of microbiology & biotechnology · 2021Review
- Reasons for 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde resistance in Saccharomyces cerevisiae: current state of knowledge and perspectives for further improvements.Applied microbiology and biotechnology · 2021Review
- Dynamic Preference for NADP/H Cofactor Binding/Release inMolecules (Basel, Switzerland) · 2021Article
- Engineering prokaryotic regulator IrrE to enhance stress tolerance in budding yeast.Biotechnology for biofuels · 2020Article
1 more citing papers are in PubMed but not listed here.
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8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pretreatments such as dilute acid at elevated temperature are effective for the hydrolysis of pentose polymers in hemicellulose and also increase the access of enzymes to cellulose fibers. However, the fermentation of resulting syrups is hindered by minor reaction products such as furfural from pentose dehydration. To mitigate this problem, four genetic traits have been identified that increase furfural tolerance in ethanol-producing Escherichia coli LY180 (strain W derivative): increased expression of fucO, ucpA, or pntAB and deletion of yqhD. Plasmids and integrated strains were used to characterize epistatic interactions among traits and to identify the most effective combinations. Furfural resistance traits were subsequently integrated into the chromosome of LY180 to construct strain XW129 (LY180 ΔyqhD ackA::PyadC'fucO-ucpA) for ethanol. This same combination of traits was also constructed in succinate biocatalysts (Escherichia coli strain C derivatives) and found to increase furfural tolerance. Strains engineered for resistance to furfural were also more resistant to the mixture of inhibitors in hemicellulose hydrolysates, confirming the importance of furfural as an inhibitory component. With resistant biocatalysts, product yields (ethanol and succinate) from hemicellulose syrups were equal to control fermentations in laboratory media without inhibitors. The combination of genetic traits identified for the production of ethanol (strain W derivative) and succinate (strain C derivative) may prove useful for other renewable chemicals from lignocellulosic sugars.
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